Adsorption and conformational modification of fibronectin and fibrinogen adsorbed on hydroxyapatite. A QCM-D study

Hydroxyapatite is a bioactive ceramic frequently used for bone engineering/replacement. One of the parameters that influence the biological response to implanted materials is the conformation of the first adsorbed protein layer. In this work, the adsorption and conformational changes of two fibroid...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2016-10, Vol.104 (10), p.2585-2594
Main Authors: Fernandez-Montes Moraleda, Belen, San Roman, Julio, Rodriguez-Lorenzo, Luis M
Format: Article
Language:English
Subjects:
Adsorption
Apatite
Biomedical materials
Bone Substitutes - chemistry
Bones
Durapatite - chemistry
fibrinogen
Fibrinogen - chemistry
fibronectin
Fibronectins - chemistry
Humans
Hydroxyapatite
Microbalances
protein adsorption
Protein Conformation
Quartz Crystal Microbalance Techniques
quartz crystal microbalance with dissipation
Surface chemistry
Surface Properties
Surgical implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hydroxyapatite is a bioactive ceramic frequently used for bone engineering/replacement. One of the parameters that influence the biological response to implanted materials is the conformation of the first adsorbed protein layer. In this work, the adsorption and conformational changes of two fibroid serum proteins; fibronectin and fibrinogen adsorbed onto four different hydroxyapatite powders are studied with a Quartz Crystal Microbalance with Dissipation (QCM‐D). Each of the calcined apatites adsorbs less protein than their corresponding synthesized samples. Adsorption on synthesized samples yields always an extended conformation whereas a reorganization of the layer is observed for the calcined samples. Fg acquires a “Side on” conformation in all the samples at the beginning of the experiment except for one of the synthesized samples where an “End‐on” conformation is obtained during the whole experiment. The Extended conformation is the active conformation for Fn. This conformation is favored by apatites with large specific surface area (SSA) and on highly concentrated media. Apatite surface features should be considered in the selection or design of materials for bone regeneration, since it is possible to control the conformation mode of attachment of Fn and Fg by an appropriate selection of them. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2585–2594, 2016.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.35802