Methods for the Analysis of Protein Phosphorylation-Mediated Cellular Signaling Networks

Protein phosphorylation-mediated cellular signaling networks regulate almost all aspects of cell biology, including the responses to cellular stimulation and environmental alterations. These networks are highly complex and comprise hundreds of proteins and potentially thousands of phosphorylation si...

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Bibliographic Details
Published in:Annual review of analytical chemistry (Palo Alto, Calif.) Calif.), 2016-06, Vol.9 (1), p.295-315
Main Authors: White, Forest M, Wolf-Yadlin, Alejandro
Format: Article
Language:English
Subjects:
Animals
Biology
Cellular communication
Control
Dynamic response
Humans
Networks
PhosphoFlow cytometry
phosphoproteomics
Phosphorylation
phosphorylation motif
Protein Binding
Protein Conformation
Proteins
Proteins - analysis
Proteins - metabolism
Signal Transduction
signaling networks
Stimulation
targeted approaches
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Summary:Protein phosphorylation-mediated cellular signaling networks regulate almost all aspects of cell biology, including the responses to cellular stimulation and environmental alterations. These networks are highly complex and comprise hundreds of proteins and potentially thousands of phosphorylation sites. Multiple analytical methods have been developed over the past several decades to identify proteins and protein phosphorylation sites regulating cellular signaling, and to quantify the dynamic response of these sites to different cellular stimulation. Here we provide an overview of these methods, including the fundamental principles governing each method, their relative strengths and weaknesses, and some examples of how each method has been applied to the analysis of complex signaling networks. When applied correctly, each of these techniques can provide insight into the topology, dynamics, and regulation of protein phosphorylation signaling networks.
ISSN:1936-1327
1936-1335
DOI:10.1146/annurev-anchem-071015-041542