Water soluble fluorophore-carbazole–Au–DNA nanohybrid: enhanced two-photon absorption for living cell imaging application

A type of water-soluble Au based nanohybrid is constructed through a D- pi -A (D = donor, A = acceptor) fluorophore-carbazole derivative (abbreviated as L) coupled with gold nanoparticles (NPs) under the guidance of calf thymus DNA (ct-DNA). The coupling interactions between components at the interf...

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Bibliographic Details
Published in:RSC advances 2015-11, Vol.5 (114), p.94446-94455
Main Authors: Zhu, Shu-Juan, Kong, Lin, Wang, Hui, Tian, Yu-Peng, Li, Sheng-Li, Xue, Zhao-Ming, Xu, Xian-Yun, Yang, Jia-Xiang
Format: Article
Language:English
Subjects:
Absorption
Cells (biology)
Coupling
Fluorescence
Gold
Imaging
Nanoparticles
Nanostructure
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Summary:A type of water-soluble Au based nanohybrid is constructed through a D- pi -A (D = donor, A = acceptor) fluorophore-carbazole derivative (abbreviated as L) coupled with gold nanoparticles (NPs) under the guidance of calf thymus DNA (ct-DNA). The coupling interactions between components at the interface increase the strength of donor-acceptor pair within the nanohybrid, which results in an energy transfer process and further brings about a dramatic blue-shifted single-photon absorption (about 53 nm from 450 nm for L to 397 nm for the hybrid) and fluorescence ( similar to 77 nm blue-shifted), decreased FL lifetime (1.65 ns for L, 0.53 ns for the hybrid), and blue-shifted two-photon excited fluorescence (2PEF, 638 nm for L, 574 nm for the hybrid). The coupling effect amongst the nanohybrid also leads to enhancement of nonlinear optical properties, including the two-photon absorption (TPA) cross section ( delta , 137.60 GM for L, 651.07 GM for the hybrid) and two-photon absorption coefficient ( beta , from 0.003669 cm GW-1 to 0.01401 cm GW-1). The two-photon fluorescence and good water solubility of the hybrid have proven to be potentially useful for two-photon microscopy imaging in living cells such as HepG2.
ISSN:2046-2069
2046-2069
DOI:10.1039/c5ra13791h