On the electromigration of charged fluorophore-labeled oligosaccharides in polyethylene oxide solutions

The separation mechanism of charged fluorophore (aminopyrenetrisulfonate)‐labeled maltooligosaccharides with α1‐4 linkages was studied in polyethylene oxide (PEO) solutions (MW 300 000 Da) with special interest to possible analyte and/or network deformations as well as potential solute–matrix intera...

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Bibliographic Details
Published in:Electrophoresis 2016-09, Vol.37 (17-18), p.2347-2351
Main Authors: Jarvas, Gabor, Kerekgyarto, Marta, Guttman, András
Format: Article
Language:English
Subjects:
Activation energy
Capillary electrophoresis
Chemical compounds
Deformation mechanisms
Electromigration
Electrophoresis, Capillary
Entanglement
Fluorescent Dyes - chemistry
Oligosaccharides - chemistry
Polyethylene Glycols - chemistry
Polyethylene oxide
Polyethylene oxides
Polymer network
Separation
Solutions
Thresholds
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Summary:The separation mechanism of charged fluorophore (aminopyrenetrisulfonate)‐labeled maltooligosaccharides with α1‐4 linkages was studied in polyethylene oxide (PEO) solutions (MW 300 000 Da) with special interest to possible analyte and/or network deformations as well as potential solute–matrix interactions. The electrophoretic mobilities of the 8‐aminopyrene‐1,3,6‐trisulfonate‐labeled maltooligosaccharides were found proportional with their MW−2/3. The Arrhenius function was used to determine the activation energy needed by the labeled sugars to migrate through the separation media. With increasing solute size, the activation energy (Ea) values decreased in polymer concentrations above the entanglement threshold of the PEO, while showed apparently independent function at the entanglement threshold. The observed phenomenon was considered as a result of solute–matrix interaction, which could be alleviated by the addition of an organic modifier to the BGE.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201600183