Rapid screening of multiple antibiotic residues in milk using disposable amperometric magnetosensors

• Published in: Analytica chimica acta 2014-04, Vol.820, p.32-38
• Main Authors: Conzuelo, F., Ruiz-Valdepeñas Montiel, V., Campuzano, S., Gamella, M., Torrente-Rodríguez, R.M., Reviejo, A.J., Pingarrón, J.M.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - chemistry; Antibiotics; Beads; Biosensing Techniques - instrumentation; Cephalosporins; Disposable Equipment; Drug Residues - analysis; Drug Residues - chemistry; Electrical measurements; Electrochemistry; Electrodes; Horseradish Peroxidase - metabolism; Magnetic beads; Magnets - chemistry; Methodology; Microspheres; Milk; Milk - chemistry; Multiplexing; Residues; Screen-printed electrodes; Sulfonamides; Tetracyclines; Time Factors
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2014.03.005

Rapid screening of multiple antibiotic residues in milk using disposable screen-printed carbon electrodes (SPCEs), a mixture of 3-target specific modified magnetic beads (MBs) and direct competitive assays using horseradish peroxidase (HRP)-labeled tracers. [Display omitted] •First disposable amperometric magnetobiosensor for screening of multiple antibiotics.•Discrimination between free and milk samples containing antibiotics at their MRLs.•Simple and short sample pretreatment and fast detection within 5min.•Useful and affordable alternative to classical assays for antibiotic screening in milk. Disposable amperometric magnetosensors, involving a mixture of modified-magnetic beads (MBs), for the multiplex screening of cephalosporins (CPHs), sulfonamides (SAs) and tetracyclines (TCs) antibiotic residues in milk are reported for the first time in this work. The multiplexed detection relies on the use of a mixture of target specific modified magnetic beads (MBs) and application of direct competitive assays using horseradish peroxidase (HRP)-labeled tracers. The amperometric responses measured at −0.20V vs. the Ag pseudo-reference electrode of screen-printed carbon electrodes (SPCE) upon the addition of H2O2 in the presence of hydroquinone (HQ) as redox mediator, were used to monitor the extent of the different affinity reactions. The developed methodology, involving a simple and short pretreatment, allowed discrimination between no contaminated UHT and raw milk samples and samples containing antibiotic residues at the maximum residue limits (MRLs). The usefulness of the multiplexed magnetosensor was demonstrated by analyzing spiked milk samples in only 5min. The results demonstrated that a clear discrimination of milk samples contaminated with antibiotics at their MRL level or their mixtures, allowing the identification of milk not complying with current legislation. These features make the developed methodology a promising alternative in the development of user-friendly devices for on-site analysis to ensure quality control for dairy products.