Cratylia mollis lectin nanoelectrode for differential diagnostic of prostate cancer and benign prostatic hyperplasia based on label-free detection

• Published in: Biosensors & bioelectronics 2016-11, Vol.85, p.171-177
• Main Authors: Silva, Priscila M.S., Lima, Amanda L.R., Silva, Bárbara V.M., Coelho, Luana C.B.B., Dutra, Rosa F., Correia, Maria T.S.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - methods; Biosensors; Cancer; Carbon nanotube; Cratylia mollis lectin; Diagnosis, Differential; Electrochemical biosensor; Electrochemical Techniques - methods; Electrodes; Fabaceae - chemistry; Fetuins - analysis; Gleason score; Humans; Immobilized Proteins - chemistry; Lectins; Limit of Detection; Male; Nanostructure; Nanotubes, Carbon - chemistry; Plant Lectins - chemistry; Poly-L-lysine; Polylysine - chemistry; Prostate; Prostate - pathology; Prostate cancer; Prostatic Hyperplasia - blood; Prostatic Hyperplasia - diagnosis; Prostatic Neoplasms - blood; Prostatic Neoplasms - diagnosis; Reproducibility of Results; Samples; Statistical analysis; Statistical methods
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.05.004

The research for new biomarkers of cancer has studied the role of fetuin glycoprotein on the metastatic disease diagnosis. Cratylia mollis is a lectin with high finity to fetuin, and used here to differentiate prostate cancer and benign prostatic hyperplasia. A label-free electrochemical nanosensor based on assembled carboxylated carbon nanotubes (COOH-CNTs) and poly-L-lysine (PLL) film was developed and applied to serum samples of prostate cancer positive for Gleason score. The electrode analytical response to fetuin in PBS samples, obtained by square wave voltammetry, exhibited a linear range from 0.5 to 25µgmL−1, with a high correlation coefficient (r=0.994, p