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Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens
Multiarray on a test strip (MATS) was developed for the detection of eight important potato pathogens. The proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. The test zone of the immunochromatographic strip comprises ordered rows of spots conta...
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| Published in: | Analytical and bioanalytical chemistry 2016-09, Vol.408 (22), p.6009-6017 |
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| container_issue | 22 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Safenkova, Irina V. Pankratova, Galina K. Zaitsev, Ilya A. Varitsev, Yuri A. Vengerov, Yuri Y. Zherdev, Anatoly V. Dzantiev, Boris B. |
| description | Multiarray on a test strip (MATS) was developed for the detection of eight important potato pathogens. The proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. The test zone of the immunochromatographic strip comprises ordered rows of spots containing antibodies specific for different potato pathogens. The assay benefits from the simplicity of immunochromatography; colored immune complexes form at the corresponding spots within the test zone. The presence and intensity of the coloration are used for identification of the target pathogens. The MATS was applied to the simultaneous detection of eight priority potato pathogens, characterized by the following limits of detection: 1 ng/mL for potato virus X and the ordinary type of potato virus Y, 10 ng/mL for potato virus M, 20 ng/mL for potato leaf roll virus, 40 ng/mL for necrotic-type potato virus Y, 100 ng/mL for potato virus S, 300 ng/mL for potato virus A, and 10
4
cells/mL for
Clavibacter michiganensis
subsp.
sepedonicus
. Analysis time was 15 min. The observed sensitivity of the MATS was comparable to the traditional enzyme-linked immunosorbent assay. The developed technique was tested on potato leaf extracts, and its efficiency for on-site control of the pathogens was confirmed in 100 % by commercial LFIA test strips.
Graphical abstract
Location of binding zones in the developed multiarray on a test strip (MATS) for simultaneous detection of eight pathogens |
| doi_str_mv | 10.1007/s00216-016-9463-6 |
| format | article |
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4
cells/mL for
Clavibacter michiganensis
subsp.
sepedonicus
. Analysis time was 15 min. The observed sensitivity of the MATS was comparable to the traditional enzyme-linked immunosorbent assay. The developed technique was tested on potato leaf extracts, and its efficiency for on-site control of the pathogens was confirmed in 100 % by commercial LFIA test strips.
Graphical abstract
Location of binding zones in the developed multiarray on a test strip (MATS) for simultaneous detection of eight pathogens</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-016-9463-6</identifier><identifier>PMID: 27007732</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Analytical Chemistry ; Antibodies ; Antibodies, Immobilized - chemistry ; Assaying ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Clavibacter michiganensis ; Diseases and pests ; Environmental monitoring ; Equipment Design ; Food Science ; Gold - chemistry ; Immunoanalysis for Environmental Monitoring and Human Health ; Immunoassay ; Immunochromatography - economics ; Immunochromatography - instrumentation ; Immunochromatography - methods ; Laboratory Medicine ; Limit of Detection ; Mats ; Metal Nanoparticles - chemistry ; Methods ; Monitoring/Environmental Analysis ; Multiplexing ; Nanoparticles ; Observations ; Onsite ; Pathogens ; Plant Diseases - virology ; Plant extracts ; Plant Viruses - isolation & purification ; Plant-pathogen relationships ; Potassium ; Potato virus A ; Potato virus M ; Potato virus S ; Potato virus X ; Potato virus Y ; Potatoes ; Protein Array Analysis - economics ; Protein Array Analysis - instrumentation ; Protein Array Analysis - methods ; Reagent Strips - analysis ; Research Paper ; Sodium ; Solanum tuberosum ; Solanum tuberosum - virology ; Spots ; Strip ; Vegetables ; Viruses</subject><ispartof>Analytical and bioanalytical chemistry, 2016-09, Vol.408 (22), p.6009-6017</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>COPYRIGHT 2016 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-7618a17c32ef9049c876a89186e31084be34d721282ed8ff896ba9c44ff7c3383</citedby><cites>FETCH-LOGICAL-c650t-7618a17c32ef9049c876a89186e31084be34d721282ed8ff896ba9c44ff7c3383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27007732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Safenkova, Irina V.</creatorcontrib><creatorcontrib>Pankratova, Galina K.</creatorcontrib><creatorcontrib>Zaitsev, Ilya A.</creatorcontrib><creatorcontrib>Varitsev, Yuri A.</creatorcontrib><creatorcontrib>Vengerov, Yuri Y.</creatorcontrib><creatorcontrib>Zherdev, Anatoly V.</creatorcontrib><creatorcontrib>Dzantiev, Boris B.</creatorcontrib><title>Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Multiarray on a test strip (MATS) was developed for the detection of eight important potato pathogens. The proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. The test zone of the immunochromatographic strip comprises ordered rows of spots containing antibodies specific for different potato pathogens. The assay benefits from the simplicity of immunochromatography; colored immune complexes form at the corresponding spots within the test zone. The presence and intensity of the coloration are used for identification of the target pathogens. The MATS was applied to the simultaneous detection of eight priority potato pathogens, characterized by the following limits of detection: 1 ng/mL for potato virus X and the ordinary type of potato virus Y, 10 ng/mL for potato virus M, 20 ng/mL for potato leaf roll virus, 40 ng/mL for necrotic-type potato virus Y, 100 ng/mL for potato virus S, 300 ng/mL for potato virus A, and 10
4
cells/mL for
Clavibacter michiganensis
subsp.
sepedonicus
. Analysis time was 15 min. The observed sensitivity of the MATS was comparable to the traditional enzyme-linked immunosorbent assay. The developed technique was tested on potato leaf extracts, and its efficiency for on-site control of the pathogens was confirmed in 100 % by commercial LFIA test strips.
Graphical abstract
Location of binding zones in the developed multiarray on a test strip (MATS) for simultaneous detection of eight pathogens</description><subject>Acids</subject><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Antibodies, Immobilized - chemistry</subject><subject>Assaying</subject><subject>Biochemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Clavibacter michiganensis</subject><subject>Diseases and pests</subject><subject>Environmental monitoring</subject><subject>Equipment Design</subject><subject>Food Science</subject><subject>Gold - chemistry</subject><subject>Immunoanalysis for Environmental Monitoring and Human Health</subject><subject>Immunoassay</subject><subject>Immunochromatography - economics</subject><subject>Immunochromatography - instrumentation</subject><subject>Immunochromatography - methods</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Mats</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Multiplexing</subject><subject>Nanoparticles</subject><subject>Observations</subject><subject>Onsite</subject><subject>Pathogens</subject><subject>Plant Diseases - virology</subject><subject>Plant extracts</subject><subject>Plant Viruses - isolation & purification</subject><subject>Plant-pathogen relationships</subject><subject>Potassium</subject><subject>Potato virus A</subject><subject>Potato virus M</subject><subject>Potato virus S</subject><subject>Potato virus X</subject><subject>Potato virus Y</subject><subject>Potatoes</subject><subject>Protein Array Analysis - economics</subject><subject>Protein Array Analysis - instrumentation</subject><subject>Protein Array Analysis - methods</subject><subject>Reagent Strips - analysis</subject><subject>Research Paper</subject><subject>Sodium</subject><subject>Solanum tuberosum</subject><subject>Solanum tuberosum - virology</subject><subject>Spots</subject><subject>Strip</subject><subject>Vegetables</subject><subject>Viruses</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkk9rFTEUxYMotlY_gBsJuGkXU_Nvkoy7R7EqtLiwrkNe5uaZMjMZkwz0fXvzOrWIIJQQEpLfuZybHITeUnJOCVEfMiGMyobU2QnJG_kMHVNJdcNkS54_7gU7Qq9yviWEtprKl-iIqSpXnB0jc70MJdiU7B7HCVtcIBecSwozPr3e3Hw_-4iTnUOPxwM4D3CHwzguU-yhgCuhiqLHcwoxhbLHcyy2RDzb8jPuYMqv0QtvhwxvHtYT9OPy083Fl-bq2-evF5urxlWrpVHVqaXKcQa-I6JzWkmrO6olcEq02AIXvWKUaQa99l53cms7J4T3VcQ1P0Gna905xV9L7cGMITsYBjtBXLKhmreSs1aIJ6C0VfV55JNQyjpNuoOB9_-gt3FJU-35niJCCs0rdb5SOzuACZOPJVlXRw9jcHECH-r5RigiWkHvHdBV4FLMOYE39alHm_aGEnMIgVlDYGoIzCEERlbNuwcry3aE_lHx59crwFYg16tpB-kvr_-t-hte1rnh</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Safenkova, Irina V.</creator><creator>Pankratova, Galina K.</creator><creator>Zaitsev, Ilya A.</creator><creator>Varitsev, Yuri A.</creator><creator>Vengerov, Yuri Y.</creator><creator>Zherdev, Anatoly V.</creator><creator>Dzantiev, Boris B.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens</title><author>Safenkova, Irina V. ; Pankratova, Galina K. ; Zaitsev, Ilya A. ; Varitsev, Yuri A. ; Vengerov, Yuri Y. ; Zherdev, Anatoly V. ; Dzantiev, Boris B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-7618a17c32ef9049c876a89186e31084be34d721282ed8ff896ba9c44ff7c3383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acids</topic><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Antibodies, Immobilized - chemistry</topic><topic>Assaying</topic><topic>Biochemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Clavibacter michiganensis</topic><topic>Diseases and pests</topic><topic>Environmental monitoring</topic><topic>Equipment Design</topic><topic>Food Science</topic><topic>Gold - chemistry</topic><topic>Immunoanalysis for Environmental Monitoring and Human Health</topic><topic>Immunoassay</topic><topic>Immunochromatography - economics</topic><topic>Immunochromatography - instrumentation</topic><topic>Immunochromatography - methods</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Mats</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Methods</topic><topic>Monitoring/Environmental Analysis</topic><topic>Multiplexing</topic><topic>Nanoparticles</topic><topic>Observations</topic><topic>Onsite</topic><topic>Pathogens</topic><topic>Plant Diseases - virology</topic><topic>Plant extracts</topic><topic>Plant Viruses - isolation & purification</topic><topic>Plant-pathogen relationships</topic><topic>Potassium</topic><topic>Potato virus A</topic><topic>Potato virus M</topic><topic>Potato virus S</topic><topic>Potato virus X</topic><topic>Potato virus Y</topic><topic>Potatoes</topic><topic>Protein Array Analysis - economics</topic><topic>Protein Array Analysis - instrumentation</topic><topic>Protein Array Analysis - methods</topic><topic>Reagent Strips - analysis</topic><topic>Research Paper</topic><topic>Sodium</topic><topic>Solanum tuberosum</topic><topic>Solanum tuberosum - virology</topic><topic>Spots</topic><topic>Strip</topic><topic>Vegetables</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Safenkova, Irina V.</creatorcontrib><creatorcontrib>Pankratova, Galina K.</creatorcontrib><creatorcontrib>Zaitsev, Ilya A.</creatorcontrib><creatorcontrib>Varitsev, Yuri A.</creatorcontrib><creatorcontrib>Vengerov, Yuri Y.</creatorcontrib><creatorcontrib>Zherdev, Anatoly V.</creatorcontrib><creatorcontrib>Dzantiev, Boris B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials science collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Safenkova, Irina V.</au><au>Pankratova, Galina K.</au><au>Zaitsev, Ilya A.</au><au>Varitsev, Yuri A.</au><au>Vengerov, Yuri Y.</au><au>Zherdev, Anatoly V.</au><au>Dzantiev, Boris B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>408</volume><issue>22</issue><spage>6009</spage><epage>6017</epage><pages>6009-6017</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Multiarray on a test strip (MATS) was developed for the detection of eight important potato pathogens. The proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. The test zone of the immunochromatographic strip comprises ordered rows of spots containing antibodies specific for different potato pathogens. The assay benefits from the simplicity of immunochromatography; colored immune complexes form at the corresponding spots within the test zone. The presence and intensity of the coloration are used for identification of the target pathogens. The MATS was applied to the simultaneous detection of eight priority potato pathogens, characterized by the following limits of detection: 1 ng/mL for potato virus X and the ordinary type of potato virus Y, 10 ng/mL for potato virus M, 20 ng/mL for potato leaf roll virus, 40 ng/mL for necrotic-type potato virus Y, 100 ng/mL for potato virus S, 300 ng/mL for potato virus A, and 10
4
cells/mL for
Clavibacter michiganensis
subsp.
sepedonicus
. Analysis time was 15 min. The observed sensitivity of the MATS was comparable to the traditional enzyme-linked immunosorbent assay. The developed technique was tested on potato leaf extracts, and its efficiency for on-site control of the pathogens was confirmed in 100 % by commercial LFIA test strips.
Graphical abstract
Location of binding zones in the developed multiarray on a test strip (MATS) for simultaneous detection of eight pathogens</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27007732</pmid><doi>10.1007/s00216-016-9463-6</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical and bioanalytical chemistry, 2016-09, Vol.408 (22), p.6009-6017 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835632544 |
| source | Springer Nature |
| subjects | Acids Analytical Chemistry Antibodies Antibodies, Immobilized - chemistry Assaying Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Clavibacter michiganensis Diseases and pests Environmental monitoring Equipment Design Food Science Gold - chemistry Immunoanalysis for Environmental Monitoring and Human Health Immunoassay Immunochromatography - economics Immunochromatography - instrumentation Immunochromatography - methods Laboratory Medicine Limit of Detection Mats Metal Nanoparticles - chemistry Methods Monitoring/Environmental Analysis Multiplexing Nanoparticles Observations Onsite Pathogens Plant Diseases - virology Plant extracts Plant Viruses - isolation & purification Plant-pathogen relationships Potassium Potato virus A Potato virus M Potato virus S Potato virus X Potato virus Y Potatoes Protein Array Analysis - economics Protein Array Analysis - instrumentation Protein Array Analysis - methods Reagent Strips - analysis Research Paper Sodium Solanum tuberosum Solanum tuberosum - virology Spots Strip Vegetables Viruses |
| title | Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens |
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