Loading…
Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell
[Display omitted] •Low-invasive and fine virtual electrode is generated using focued low energy electron beam (EB).•The virtual electode induecs local detachment of nanopartilces, and induced retraction of myoblast cell due to prestress.•Distribution of prestressed mechanical strain of the cell were...
Saved in:
| Published in: | Sensors and actuators. B, Chemical Chemical, 2016-11, Vol.236, p.659-667 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3 |
| container_end_page | 667 |
| container_issue | |
| container_start_page | 659 |
| container_title | Sensors and actuators. B, Chemical |
| container_volume | 236 |
| creator | Hoshino, Takayuki Miyazako, Hiroki Nakayama, Atsuki Wagatsuma, Akira Mabuchi, Kunihiko |
| description | [Display omitted]
•Low-invasive and fine virtual electrode is generated using focued low energy electron beam (EB).•The virtual electode induecs local detachment of nanopartilces, and induced retraction of myoblast cell due to prestress.•Distribution of prestressed mechanical strain of the cell were visualied and this indicated the elastic strain energy was stored arownd the nucleus and the lamellipodium.•The spatial resolution of the viratual electrode was ∼120-nm wide, and the repultion force was affected in |
| doi_str_mv | 10.1016/j.snb.2016.06.023 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835637944</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400516308759</els_id><sourcerecordid>1835637944</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMoWKs_wFuOXnadJPuJJyn1Awpe9CaEbDLRlN1sTbaF_ntT1rMwMAPzvsM7DyG3DHIGrLrf5tF3OU9jDqm4OCML1tQiE1DX52QBLS-zAqC8JFcxbgGgEBUsyOe6Rz2F0dMO1UCdN3uNhlrnkR5cmPaqpzhLDFI7Bjqg_lbe6bSIU1DO08HpMEY97o50tLR3B-e_qMa-vyYXVvURb_76knw8rd9XL9nm7fl19bjJtGirKdNa1JZbywsjsLBtU5mq7AwvClBVhYo3lrEWRGktNMJqbmzXWOh0bdqGWxRLcjff3YXxZ49xkoOLpwDK47iPkjWirETdFkWSsll6ihwDWrkLblDhKBnIE0m5lYmkPJGUkIqL5HmYPZh-ODgMMmqHPnFyIZGRZnT_uH8BWNB9iw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835637944</pqid></control><display><type>article</type><title>Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell</title><source>Elsevier</source><creator>Hoshino, Takayuki ; Miyazako, Hiroki ; Nakayama, Atsuki ; Wagatsuma, Akira ; Mabuchi, Kunihiko</creator><creatorcontrib>Hoshino, Takayuki ; Miyazako, Hiroki ; Nakayama, Atsuki ; Wagatsuma, Akira ; Mabuchi, Kunihiko</creatorcontrib><description>[Display omitted]
•Low-invasive and fine virtual electrode is generated using focued low energy electron beam (EB).•The virtual electode induecs local detachment of nanopartilces, and induced retraction of myoblast cell due to prestress.•Distribution of prestressed mechanical strain of the cell were visualied and this indicated the elastic strain energy was stored arownd the nucleus and the lamellipodium.•The spatial resolution of the viratual electrode was ∼120-nm wide, and the repultion force was affected in <1μm in redius.
We have demonstrated nanomechanical applications using physicochemical and electrochemical phenomena of inverted-electron beam lithography (I-EBL), which induced in-situ two-dimensional (2-D) processing on wet samples and a living cell after the EB was stoppted in a 100-nm thick SiN membrane. The incident EB generates a virtual electrode and then this induces electrochemical and electrokinetic phenomena around the scanning trajectory. The I-EBL processing has a 120-nm resolution in full-width-at-half-maximum (FWHM) at the deposited line-and-space pattern in 10mM 3,4-ethylenedioxythiophene (EDOT) diluted water solution. The virtual electrode also causes a electrokinetic local repulsive force with <∼1μm resolution toward the negatively charged nanoparticles, and the 2-D scanning of the EB allows 2-D actuation of the nanoparticles dispersed in a pure water solution. The virtual electrode also induces local detachment of adherent nanoparticles and focal adhesion of a living cell from the SiN membrane in a saline solution, probably due to both electrokinetic and partly chemical protein denaturation processes. The local detachment of a living cell is utilized to investigate spatio-temporal distributions of intracellular elastic strain as mechanical strain microscopy (MSM), which represents mechanical connectivity in the intracellular structure. This MSM should provide visualization of the location of the force generation in the cell.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2016.06.023</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Biomechanical analysis ; Cells (biology) ; Detachment ; Electrochemical phenomenon ; Electrodes ; Electrokinetics ; Electron-beam ; Membranes ; Microscopy ; Nano manipulation ; Nanoparticles ; Single cell analysis ; Strain ; Virtual electrode</subject><ispartof>Sensors and actuators. B, Chemical, 2016-11, Vol.236, p.659-667</ispartof><rights>2016 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3</citedby><cites>FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3</cites><orcidid>0000-0003-4376-7853</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Hoshino, Takayuki</creatorcontrib><creatorcontrib>Miyazako, Hiroki</creatorcontrib><creatorcontrib>Nakayama, Atsuki</creatorcontrib><creatorcontrib>Wagatsuma, Akira</creatorcontrib><creatorcontrib>Mabuchi, Kunihiko</creatorcontrib><title>Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell</title><title>Sensors and actuators. B, Chemical</title><description>[Display omitted]
•Low-invasive and fine virtual electrode is generated using focued low energy electron beam (EB).•The virtual electode induecs local detachment of nanopartilces, and induced retraction of myoblast cell due to prestress.•Distribution of prestressed mechanical strain of the cell were visualied and this indicated the elastic strain energy was stored arownd the nucleus and the lamellipodium.•The spatial resolution of the viratual electrode was ∼120-nm wide, and the repultion force was affected in <1μm in redius.
We have demonstrated nanomechanical applications using physicochemical and electrochemical phenomena of inverted-electron beam lithography (I-EBL), which induced in-situ two-dimensional (2-D) processing on wet samples and a living cell after the EB was stoppted in a 100-nm thick SiN membrane. The incident EB generates a virtual electrode and then this induces electrochemical and electrokinetic phenomena around the scanning trajectory. The I-EBL processing has a 120-nm resolution in full-width-at-half-maximum (FWHM) at the deposited line-and-space pattern in 10mM 3,4-ethylenedioxythiophene (EDOT) diluted water solution. The virtual electrode also causes a electrokinetic local repulsive force with <∼1μm resolution toward the negatively charged nanoparticles, and the 2-D scanning of the EB allows 2-D actuation of the nanoparticles dispersed in a pure water solution. The virtual electrode also induces local detachment of adherent nanoparticles and focal adhesion of a living cell from the SiN membrane in a saline solution, probably due to both electrokinetic and partly chemical protein denaturation processes. The local detachment of a living cell is utilized to investigate spatio-temporal distributions of intracellular elastic strain as mechanical strain microscopy (MSM), which represents mechanical connectivity in the intracellular structure. This MSM should provide visualization of the location of the force generation in the cell.</description><subject>Biomechanical analysis</subject><subject>Cells (biology)</subject><subject>Detachment</subject><subject>Electrochemical phenomenon</subject><subject>Electrodes</subject><subject>Electrokinetics</subject><subject>Electron-beam</subject><subject>Membranes</subject><subject>Microscopy</subject><subject>Nano manipulation</subject><subject>Nanoparticles</subject><subject>Single cell analysis</subject><subject>Strain</subject><subject>Virtual electrode</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKs_wFuOXnadJPuJJyn1Awpe9CaEbDLRlN1sTbaF_ntT1rMwMAPzvsM7DyG3DHIGrLrf5tF3OU9jDqm4OCML1tQiE1DX52QBLS-zAqC8JFcxbgGgEBUsyOe6Rz2F0dMO1UCdN3uNhlrnkR5cmPaqpzhLDFI7Bjqg_lbe6bSIU1DO08HpMEY97o50tLR3B-e_qMa-vyYXVvURb_76knw8rd9XL9nm7fl19bjJtGirKdNa1JZbywsjsLBtU5mq7AwvClBVhYo3lrEWRGktNMJqbmzXWOh0bdqGWxRLcjff3YXxZ49xkoOLpwDK47iPkjWirETdFkWSsll6ihwDWrkLblDhKBnIE0m5lYmkPJGUkIqL5HmYPZh-ODgMMmqHPnFyIZGRZnT_uH8BWNB9iw</recordid><startdate>20161129</startdate><enddate>20161129</enddate><creator>Hoshino, Takayuki</creator><creator>Miyazako, Hiroki</creator><creator>Nakayama, Atsuki</creator><creator>Wagatsuma, Akira</creator><creator>Mabuchi, Kunihiko</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0003-4376-7853</orcidid></search><sort><creationdate>20161129</creationdate><title>Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell</title><author>Hoshino, Takayuki ; Miyazako, Hiroki ; Nakayama, Atsuki ; Wagatsuma, Akira ; Mabuchi, Kunihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biomechanical analysis</topic><topic>Cells (biology)</topic><topic>Detachment</topic><topic>Electrochemical phenomenon</topic><topic>Electrodes</topic><topic>Electrokinetics</topic><topic>Electron-beam</topic><topic>Membranes</topic><topic>Microscopy</topic><topic>Nano manipulation</topic><topic>Nanoparticles</topic><topic>Single cell analysis</topic><topic>Strain</topic><topic>Virtual electrode</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoshino, Takayuki</creatorcontrib><creatorcontrib>Miyazako, Hiroki</creatorcontrib><creatorcontrib>Nakayama, Atsuki</creatorcontrib><creatorcontrib>Wagatsuma, Akira</creatorcontrib><creatorcontrib>Mabuchi, Kunihiko</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoshino, Takayuki</au><au>Miyazako, Hiroki</au><au>Nakayama, Atsuki</au><au>Wagatsuma, Akira</au><au>Mabuchi, Kunihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2016-11-29</date><risdate>2016</risdate><volume>236</volume><spage>659</spage><epage>667</epage><pages>659-667</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>[Display omitted]
•Low-invasive and fine virtual electrode is generated using focued low energy electron beam (EB).•The virtual electode induecs local detachment of nanopartilces, and induced retraction of myoblast cell due to prestress.•Distribution of prestressed mechanical strain of the cell were visualied and this indicated the elastic strain energy was stored arownd the nucleus and the lamellipodium.•The spatial resolution of the viratual electrode was ∼120-nm wide, and the repultion force was affected in <1μm in redius.
We have demonstrated nanomechanical applications using physicochemical and electrochemical phenomena of inverted-electron beam lithography (I-EBL), which induced in-situ two-dimensional (2-D) processing on wet samples and a living cell after the EB was stoppted in a 100-nm thick SiN membrane. The incident EB generates a virtual electrode and then this induces electrochemical and electrokinetic phenomena around the scanning trajectory. The I-EBL processing has a 120-nm resolution in full-width-at-half-maximum (FWHM) at the deposited line-and-space pattern in 10mM 3,4-ethylenedioxythiophene (EDOT) diluted water solution. The virtual electrode also causes a electrokinetic local repulsive force with <∼1μm resolution toward the negatively charged nanoparticles, and the 2-D scanning of the EB allows 2-D actuation of the nanoparticles dispersed in a pure water solution. The virtual electrode also induces local detachment of adherent nanoparticles and focal adhesion of a living cell from the SiN membrane in a saline solution, probably due to both electrokinetic and partly chemical protein denaturation processes. The local detachment of a living cell is utilized to investigate spatio-temporal distributions of intracellular elastic strain as mechanical strain microscopy (MSM), which represents mechanical connectivity in the intracellular structure. This MSM should provide visualization of the location of the force generation in the cell.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2016.06.023</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-4376-7853</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-4005 |
| ispartof | Sensors and actuators. B, Chemical, 2016-11, Vol.236, p.659-667 |
| issn | 0925-4005 1873-3077 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835637944 |
| source | Elsevier |
| subjects | Biomechanical analysis Cells (biology) Detachment Electrochemical phenomenon Electrodes Electrokinetics Electron-beam Membranes Microscopy Nano manipulation Nanoparticles Single cell analysis Strain Virtual electrode |
| title | Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T19%3A04%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electron%20beam%20induced%20fine%20virtual%20electrode%20for%20mechanical%20strain%20microscopy%20of%20living%20cell&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Hoshino,%20Takayuki&rft.date=2016-11-29&rft.volume=236&rft.spage=659&rft.epage=667&rft.pages=659-667&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2016.06.023&rft_dat=%3Cproquest_cross%3E1835637944%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c396t-cc37f2ff24d3e4f986d65bd2440a66ea28f119035ff083fc2dfb8f0bc7d982fe3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1835637944&rft_id=info:pmid/&rfr_iscdi=true |