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Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe

•We have successfully synthesized a near-infrared fluorescent probe, named PDP-Cy5, realizing visualization of DNA and RNA G-quadruplexes.•PDP-Cy5 had excellent selectivity for G-quadruplexes, eliminating interference of other structures of nucleic acids.•PDP-Cy5 also performed surprising ability to...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2016-11, Vol.236, p.268-275
Main Authors: Wu, Fan, Liu, Chaoxing, Chen, Yuqi, Yang, Shixi, Xu, Jiahui, Huang, Rong, Wang, Xiang, Li, Manjia, Liu, Wenting, Mao, Wuxiang, Zhou, Xiang
Format: Article
Language:English
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Summary:•We have successfully synthesized a near-infrared fluorescent probe, named PDP-Cy5, realizing visualization of DNA and RNA G-quadruplexes.•PDP-Cy5 had excellent selectivity for G-quadruplexes, eliminating interference of other structures of nucleic acids.•PDP-Cy5 also performed surprising ability to stabilize G-quadruplexes. G-quadruplexes, one of the most significant secondary structure of nucleic acid, are formed by stacking G quartets, which received broad interests due to their involvement in telomere function, gene transcription and recombination. As for ligand, better selectivity for G-quadruplex against other DNA or RNA structures and higher ability to stabilize G-quadruplex are necessary. In addition, developing a new probe to recognize G-quadruplex is desired for utilizing G-quadruplex structure to relevant biological processes. In this study, we report a Cy5 labelled ligand (named PDP-Cy5) developed as a near-infrared fluorescent ligand for G-quadruplexes both in DNA and RNA. The results indicated that PDP-Cy5 selectively induced the formation of intramolecular G-quadruplexes with strong binding affinity. Furthermore, this Cy5 labelled ligand can effectively stabilize G-quadruplexes. Moreover, the direct visualization of G-quadruplexes in gel, even on cell level is realized by using PDP-Cy5.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.05.162