Cytotoxicity assessment of adipose-derived mesenchymal stem cells on synthesized biodegradable Mg-Zn-Ca alloys

• Published in: Materials Science & Engineering C 2016-12, Vol.69, p.584-597
• Main Authors: Fazel Anvari-Yazdi, Abbas, Tahermanesh, Kobra, Hadavi, Seyed Mohammad Mehdi, Talaei-Khozani, Tahereh, Razmkhah, Mahboobeh, Abed, Seyedeh Mehr, Mohtasebi, Maryam Sadat
• Format: Article
• Language: English
• Subjects: Adipose stem cells; Adipose Tissue - cytology; Alloys; Alloys - pharmacology; Antigens, CD - metabolism; Biocompatibility; Biocompatible Materials - pharmacology; Biodegradability; Biodegradation; Biomaterials; Biomedical materials; Bone tissue; Calcium - pharmacology; Cell Death - drug effects; Cell Shape - drug effects; Cells, Cultured; Corrosion; Cytotoxicity; Electrochemistry; Female; Flow Cytometry; Humans; Hydrogen-Ion Concentration; Magnesium - pharmacology; Magnesium alloy; Magnesium base alloys; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - ultrastructure; Molecular Weight; Osmolar Concentration; Spectrometry, X-Ray Emission; Spectroscopy, Fourier Transform Infrared; Surface Properties; Surgical implants; Viability; Zinc - pharmacology
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2016.07.016

Magnesium (Mg)-based alloys have been extensively considered as biodegradable implant materials for orthopedic surgery. Mg and its alloys are metallic biomaterials that can degrade in the body and promote new bone formation. In this study, the corrosion behavior and cytotoxicity of Mg-Zn-Ca alloys are evaluated with adipose-derived mesenchymal stem cells (ASCs). Mg-2Zn and Mg-2Zn-xCa (x=1, 2 and 3wt.%) alloys were designated. Mg alloys were analyzed with scanning electron microscopy and potentiodynamic polarization. To understand the in-vitro biocompatibility and cytotoxicity of Mg-2Zn and Mg-2Zn-xCa alloys, ASCs were cultured for 24 and 72h in contact with 10%, 50% and 100% extraction of all alloys prepared in DMEM. Cell cytotoxicity and viability of ASCs were examined by MTT assay. Alloying elements including Zn and Ca improved the corrosion resistance of alloys were compared with pure Mg. The cytotoxicity results showed that all alloys had no significant adverse effects on cell viability in 24h. After 72h, cell viability and proliferation increased in the cells exposed to pure Mg and Mg-2Zn-1Ca extracts. The release of Mg, Zn and Ca ions in culture media had no toxic impacts on ASCs viability and proliferation. Mg-2Zn-1Ca alloy can be suggested as a good candidate to be used in biomedical applications. [Display omitted] •Short and long term corrosion behavior of Mg-Zn-Ca alloys studied•Viability and toxicity of Adipose-derived Stem cells studied with Mg-Zn-Ca alloys•Understanding the morphology of cultured adipose stem cells on Mg alloys•Stem cells on Mg-Zn-Ca alloys could proliferate and expand