Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation

Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100-120 mg l^sup -1^ about 20 h after inoculation, but the titer drops rapidly a...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2001-12, Vol.23 (23), p.1903-1905
Main Authors: YUN, Soon-Il, YAHYA, Ahmad R. M, COSSAR, Doug, ANDERSON, William A, SCHARER, Jeno M, MOO-YOUNG, Murray
Format: Article
Language:English
Subjects:
Antineoplastic drugs. Cytokines
Bacteriology
Biological and medical sciences
Biotechnology
Fermentation
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Production of active biomolecules
Streptomyces lividans
Temperature
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100-120 mg l^sup -1^ about 20 h after inoculation, but the titer drops rapidly as the result of proteolysis. Reducing the culture temperature at this point has a dramatic effect on product yield, increasing the final concentration in the supernatant at least two-fold.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1013798129637