Arrayed water-in-oil droplet bilayers for membrane transport analysis

The water-in-oil droplet bilayer is a simple and useful lipid bilayer system for membrane transport analysis. The droplet interface bilayer is readily formed by the contact of two water-in-oil droplets enwrapped by a phospholipid monolayer. However, the size of individual droplets with femtoliter vo...

Full description

Saved in:
Bibliographic Details
Published in:Lab on a chip 2016-01, Vol.16 (16), p.3043-3048
Main Authors: Watanabe, R, Soga, N, Hara, M, Noji, H
Format: Article
Language:English
Subjects:
Algorithms
Arrays
Bacterial Toxins - metabolism
Biological Transport
Calcium Chloride - metabolism
Coloring Agents - metabolism
Contact
Devices
Droplets
Drugs
Equipment Design
Hemolysin Proteins - metabolism
High-Throughput Screening Assays - instrumentation
Hydrophobic and Hydrophilic Interactions
Lipid Bilayers - metabolism
Lipids
Membranes
Microarray Analysis - instrumentation
Models, Biological
Permeability
Phosphatidylethanolamines - metabolism
Phosphatidylglycerols - metabolism
Transport
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The water-in-oil droplet bilayer is a simple and useful lipid bilayer system for membrane transport analysis. The droplet interface bilayer is readily formed by the contact of two water-in-oil droplets enwrapped by a phospholipid monolayer. However, the size of individual droplets with femtoliter volumes in a high-throughput manner is difficult to control, resulting in low sensitivity and throughput of membrane transport analysis. To overcome this drawback, in this study, we developed a novel micro-device in which a large number of droplet interface bilayers (>500) are formed at a time by using femtoliter-sized droplet arrays immobilized on a hydrophobic/hydrophilic substrate. The droplet volume was controllable from 3.5 to 350 fL by changing the hydrophobic/hydrophilic pattern on the device, allowing high-throughput analysis of membrane transport mechanisms including membrane permeability to solutes (e.g., ions or small molecules) with or without the aid of transport proteins. Thus, this novel platform broadens the versatility of water-in-oil droplet bilayers and will pave the way for novel analytical and pharmacological applications such as drug screening.
ISSN:1473-0197
1473-0189
DOI:10.1039/c6lc00155f