Construction of photodynamic-effect immunofluorescence probes by a complex of quantum dots, immunoglobulin G and chlorin e6 and their application in HepG2 cell killing

• Published in: Luminescence (Chichester, England) England), 2016-09, Vol.31 (6), p.1174-1181
• Main Authors: Yan, Zheng-Yu, Wang, Li-Li, Fei, Meng-Ying, Liu, Xin-Ying, Su, Yi-Long, Du, Qing-Qing, Wu, Sheng-Mei
• Format: Article
• Language: English
• Subjects: Antibodies; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; CdTe quantum dots; Cell Death - drug effects; Cell Proliferation - drug effects; Chlorin e6; Covalence; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - pharmacology; Hep G2 Cells; Humans; immunofluorescence probes; Immunoglobulin G - chemistry; Immunoglobulins; Killing; MTT; PDT; Photosensitivity; Photosensitizing Agents - chemical synthesis; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; Porphyrins - chemistry; Porphyrins - pharmacology; Quantum Dots; Recognition; Structure-Activity Relationship; Tumors
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.3054

In this study, tri‐functional immunofluorescent probes (Ce6–IgG–QDs) based on covalent combinations of quantum dots (QDs), immunoglobulin G (IgG) and chlorin e6 (Ce6) were developed and their photodynamic ability to induce the death of cancer cells was demonstrated. Strategically, one type of second‐generation photosensitizer, Ce6, was first coupled with anti‐IgG antibody using the EDC/NHS cross‐linking method to construct the photosensitive immunoconjugate Ce6–IgG. Then, a complex of Ce6–IgG–QDs immunofluorescent probes was obtained in succession by covalently coupling Ce6–IgG to water soluble CdTe QDs. The as‐manufactured Ce6–IgG–QDs maintained the bio‐activities of both the antigen–antibody‐based tumour targeting effects of IgG and the photodynamic‐related anticancer activities of Ce6. By way of polyclonal antibody interaction with rabbit anti‐human epidermal growth factor receptor (anti‐EGFR antibody, N‐terminus), Ce6–IgG–QDs were labelled indirectly onto the surface of human hepatocarcinoma (HepG2) cells in cell recognition and killing experiments. The results indicated that the Ce6–IgG–QDs probes have excellent tumour cell selectivity and higher photosensitivity in photodynamic therapy (PDT) compared with Ce6 alone, due to their antibody‐based specific recognition and location of HepG2 cells and the photodynamic effects of Ce6 killed cells based on efficient fluorescence resonance energy transfer between QDs and Ce6. Copyright © 2015 John Wiley & Sons, Ltd.