Rapid Identification and Comparison of Compounds with Antioxidant Activity in Coreopsis tinctoria Herbal Tea by High-Performance Thin-Layer Chromatography Coupled with DPPH Bioautography and Densitometry

• Published in: Journal of food science 2016-09, Vol.81 (9), p.C2218-C2223
• Main Authors: Lam, Shing-Chung, Lam, Sio-Fong, Zhao, Jing, Li, Shao-Ping
• Format: Article
• Language: English
• Subjects: antioxidant activity; Antioxidants; Antioxidants - analysis; Antioxidants - pharmacology; Biological activity; Biomarkers; Biphenyl Compounds - metabolism; Chalcones - analysis; Chalcones - pharmacology; Chemometrics; Chlorogenic acid; Chlorogenic Acid - analogs & derivatives; Chlorogenic Acid - analysis; Chlorogenic Acid - pharmacology; Chromatography; Chromatography, Thin Layer - methods; Chrysanthemum; Chrysanthemum - chemistry; Chrysanthemum morifolium; Cluster analysis; Clustering; Coreopsis - chemistry; Coreopsis tinctoria; Densitometers; Densitometry; Ethanol; Ethyl alcohol; Flavanones - analysis; Flavanones - pharmacology; Functional foods & nutraceuticals; Herbal tea; Herbs; HPTLC-DPPH; identification; Pharmacology; Picrates - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Principal components analysis; Quality Control; Quinic Acid - analogs & derivatives; Quinic Acid - analysis; Quinic Acid - pharmacology; Scanning; Supermarkets; Tea; Teas, Herbal - analysis; Thin layer chromatography
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.13402

A simple and efficient method based on high‐performance thin‐layer chromatography coupled with 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) bioautography (HPTLC‐DPPH) was established for the screening and comparison of antioxidants in different parts of Coreopsis tinctoria herbal tea from different origins and other related herbal tea materials, which used Chrysanthemum morifolium cv. “Gongju” and “Hangju” in this study. Scanning densitometry after DPPH derivatization was applied for the determination of antioxidant capacities of isolated compounds in each sample. It is considered that ethanol extracts of C. tinctoria had stronger antioxidant activity and more characteristic bands than those of 2 compared samples, C. morifolium cv. “Gongju” and “Hangju.” Chemometric analysis results showed that the combination of hierarchical clustering analysis and principal component analysis based on determined antioxidant capacities could be used for the discrimination of different parts of C. tinctoria and C. morifolium. Results showed that 7 compounds made up the major contributions of antioxidant activity in C. tinctoria, including okanin, isookanin, marein, flavanomarein, 5,7,3′,5′‐tetrahydroxyflavanone‐7‐O‐glucoside, 3,5‐dicaffeoylquinic acid, and chlorogenic acid. Therefore, 7 compounds were identified as major antioxidant biomarkers for quality control of C. tinctoria. Results demonstrated that the established method could be applied for the identification of C. tinctoria, and were beneficial for the bioactivity‐based quality control of C. tinctoria. Practical Applications In recent years, Coreopsis tinctoria has become a popular herbal tea material in the functional food market because of its remarkable pharmacological activities. The study introduced a simple and rapid identification method for C. tinctoria based on HPTLC and DPPH bioautography. The HPTLC‐DPPH method coupled with scanning densitometry could be used for comparison of different samples related to C. tinctoria. The antioxidant activity of C. tinctoria was compared with commonly used herbal tea materials, Chrysanthemum morifolium cv. “Gongju” and “Hangju,” and the results suggested that C. tinctoria had higher antioxidant potentials.