Cardioprotection by Remote Ischemic Preconditioning is Blocked in the Aged Rat Heart in Vivo

Objectives In animal studies, remote ischemic preconditioning (RIPC) is a powerful tool to protect the heart from ischemia and reperfusion injury. Unfortunately, this effect was not seen consistently in recent large clinical trials. Aging was shown to be a confounding factor for the effect of direct...

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Bibliographic Details
Published in:Journal of cardiothoracic and vascular anesthesia 2017-08, Vol.31 (4), p.1223-1226
Main Authors: Behmenburg, Friederike, MD, Heinen, André, MD, PhD, Bruch, Lilli vom, MD, Hollmann, Markus W., MD, PhD, DEAA, Huhn, Ragnar, MD, PhD
Format: Article
Language:English
Subjects:
aging
Aging - pathology
Aging - physiology
Anesthesia & Perioperative Care
Animals
conditioning
Critical Care
Heart - physiopathology
Ischemic Preconditioning, Myocardial - methods
Male
myocardial infarction
Myocardial Reperfusion Injury - physiopathology
Myocardial Reperfusion Injury - prevention & control
Rats
Rats, Wistar
remote ischemic preconditioning
reperfusion injury
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Summary:Objectives In animal studies, remote ischemic preconditioning (RIPC) is a powerful tool to protect the heart from ischemia and reperfusion injury. Unfortunately, this effect was not seen consistently in recent large clinical trials. Aging was shown to be a confounding factor for the effect of direct preconditioning in experimental studies, but whether aging also can influence the effect of RIPC and thus be responsible for the contradictory clinical effect is unknown. The aim of this study was to investigate whether the cardioprotective effect of RIPC was abolished by aging. Design Randomized, prospective, blinded laboratory investigation. Setting Experimental laboratory. Participants Male Wistar rats. Interventions Anesthetized young (Y, 2-3 months) and aged (A, 22-24 months) male Wistar rats were randomized to 4 groups (n = 6 per group). Control animals (Y-Con and A-Con) were not treated further; RIPC groups (Y-RIPC and A-RIPC) received 4 cycles of 5 minutes of bilateral hind limb ischemia interspersed with 5 minutes reperfusion before myocardial ischemia and reperfusion. All animals underwent 25 minutes of regional myocardial ischemia and 120 minutes of reperfusion. At the end of reperfusion, infarct size was determined by TTC staining. Measurements and Main Results In the control group of young rats, infarct size was 56±9% of the area at risk. RIPC reduced infarct size to 31±9% (p
ISSN:1053-0770
1532-8422
DOI:10.1053/j.jvca.2016.07.005