Macrophage polarisation affects their regulation of trophoblast behaviour

• Published in: Placenta (Eastbourne) 2016-11, Vol.47, p.73-80
• Main Authors: Buckley, R.J, Whitley, G.S, Dumitriu, I.E, Cartwright, J.E
• Format: Article
• Language: English
• Subjects: Caspase 3 - metabolism; Cell Line; Cell Polarity - physiology; Culture Media, Conditioned; Decidua; Decidua - cytology; Decidua - metabolism; Extravillous trophoblast; Female; Humans; Internal Medicine; Macrophage; Macrophages - cytology; Macrophages - metabolism; Obstetrics and Gynecology; Polarisation; Pregnancy; Pregnancy Trimester, First; THP-1; Trophoblasts - cytology; Trophoblasts - metabolism
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2016.09.004

Abstract Introduction During the first trimester of human pregnancy, fetally-derived extravillous trophoblast (EVT) cells invade into uterine decidua and remodel the uterine spiral arteries to ensure that sufficient blood reaches the maternal-fetal interface. Decidual macrophages have been implicated in the regulation of decidual remodelling and aberrant activation of these immune cells is associated with pre-eclampsia. Methods The monocytic cell line THP-1 was activated to induce an M1 or M2 phenotype and the conditioned media was used to treat the EVT cell line SGHPL-4 in order to determine the effect of macrophage polarisation on trophoblast behaviour in-vitro . SGHPL-4 cell functions were assessed using time-lapse microscopy, endothelial-like tube formation assays and western blot. Results The polarisation state of the THP-1 cells was found to differentially alter the behaviour of trophoblast cells in-vitro with pro-inflammatory M1 conditioned media significantly inhibiting trophoblast motility, impeding trophoblast tube formation, and inducing trophoblast expression of caspase 3, when compared to anti-inflammatory M2 conditioned media. Discussion Macrophages can regulate trophoblast functions that are critical during decidual remodelling in early pregnancy. Importantly, there is differential regulation of trophoblast function in response to the polarisation state of these cells. Our studies indicate that the balance between a pro- and anti-inflammatory environment is important in regulating the cellular interactions at the maternal-fetal interface and that disturbances in this balance likely contribute to pregnancy disorders associated with poor trophoblast invasion and vessel remodelling.