Diagnostic Algorithm for Detection of Targetable Driver Mutations in Lung Adenocarcinomas: Comprehensive Analyses of 205 Cases with Immunohistochemistry, Real-time PCR and Fluorescence In Situ Hybridization Methods

Highlights • EGFR mutation, ALK and ROS1 rearrangements are tested in 205 lung adenocarcinomas. • Immunohistochemistry for EGFR mutation shows low sensitivity but high specificity. • Antibody SP125 presents a higher sensitivity than 43B2 in detecting EGFR p.L858R. • Immunohistochemistry for ALK and...

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Published in:Lung cancer (Amsterdam, Netherlands) Netherlands), 2016-11, Vol.101, p.40-47
Main Authors: Kao, Hua-Lin, Yeh, Yi-Chen, Lin, Chin-Hsuan, Hsu, Wei-Fang, Hsieh, Wen-Yu, Ho, Hsiang-Ling, Chou, Teh-Ying
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Adenocarcinoma of Lung
Aged
Algorithms
Driver mutations
ErbB Receptors - genetics
Female
Gene Rearrangement
Hematology, Oncology and Palliative Medicine
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence - methods
Lung adenocarcinoma
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Male
Middle Aged
Molecular testing
Mutation
Neoplasm Staging
Prospective Studies
Protein-Tyrosine Kinases - genetics
Proto-Oncogene Proteins - genetics
Pulmonary/Respiratory
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Summary:Highlights • EGFR mutation, ALK and ROS1 rearrangements are tested in 205 lung adenocarcinomas. • Immunohistochemistry for EGFR mutation shows low sensitivity but high specificity. • Antibody SP125 presents a higher sensitivity than 43B2 in detecting EGFR p.L858R. • Immunohistochemistry for ALK and ROS1 shows high sensitivity and high specificity. • An algorithm for applying immunohistochemistry in molecular diagnosis is proposed.
ISSN:0169-5002
1872-8332
DOI:10.1016/j.lungcan.2016.09.007