Measuring Oxidative DNA Damage With 8-Hydroxy-2’-Deoxyguanosine Levels in Patients With Laryngeal Cancer

Objectives: 8-Hydroxy-2’-deoxyguanosine is a biomolecule associated with DNA damage. We evaluated oxidative stress and DNA damage in patients with laryngeal cancer by measuring 8-hydroxy-2’-deoxyguanosine levels. Methods: This study enrolled 117 subjects, including 64 controls and 53 patients who ha...

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Bibliographic Details
Published in:Annals of otology, rhinology & laryngology rhinology & laryngology, 2017-02, Vol.126 (2), p.103-109
Main Authors: Mazlumoglu, Muhammet Recai, Ozkan, Ozalkan, Alp, Hamit Hakan, Ozyildirim, Ercan, Bingol, Fatih, Yoruk, Ozgur, Kuduban, Ozan
Format: Article
Language:English
Subjects:
Carcinoma, Squamous Cell - metabolism
Case-Control Studies
Chromatography, High Pressure Liquid
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
DNA - metabolism
DNA Damage
Female
Head and Neck Neoplasms - metabolism
Humans
Laryngeal Neoplasms - metabolism
Laryngectomy
Male
Middle Aged
Oxidative Stress
Smoking - metabolism
Squamous Cell Carcinoma of Head and Neck
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Summary:Objectives: 8-Hydroxy-2’-deoxyguanosine is a biomolecule associated with DNA damage. We evaluated oxidative stress and DNA damage in patients with laryngeal cancer by measuring 8-hydroxy-2’-deoxyguanosine levels. Methods: This study enrolled 117 subjects, including 64 controls and 53 patients who had benign vocal cord lesions or laryngeal cancer. The benign excised lesions, tumor tissue, noncancerous laryngeal tissue, blood, and urine were subjected to high-performance liquid chromatography, and 8-hydroxy-2’-deoxyguanosine levels were compared between groups. Results: Blood and urine 8-hydroxy-2’-deoxyguanosine levels in patients with laryngeal carcinoma were significantly higher than in the controls (P = .00002, P = .00001). The 8-hydroxy-2’-deoxyguanosine level was significantly higher in tumor tissues than in non-tumor tissue and benign vocal cord lesion tissues (P = .00002, P = .000001). Conclusions: We determined that laryngeal cancer was associated with oxidative stress, which may be quantified by measuring 8-hydroxy-2’-deoxyguanosine. For a patient with a suspicious laryngeal lesion, 8-hydroxy-2’-deoxyguanosine levels in blood and urine can provide advance information about the likely diagnosis.
ISSN:0003-4894
1943-572X
DOI:10.1177/0003489416675873