Subcapsular Implantation of Pancreatic Islets in Syngeneic, Allogeneic, and Xenogeneic Mice

Abstract Background Reliable and reproducible transplantation is essential to the success of a number of particular investigations. Renal subcapsule is the most selected site for islet transplantation mainly owing to its easy access, readiness for retrieval, and possibility of reimplantation. Method...

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Bibliographic Details
Published in:Transplantation proceedings 2016-10, Vol.48 (8), p.2821-2825
Main Authors: Ma, S.-N, Yuan, Y.-H, Guo, X.-R, Li, D.-S
Format: Article
Language:English
Subjects:
Animals
Blood Glucose - metabolism
Bowman Capsule
Diabetes Mellitus, Experimental - blood
Diabetes Mellitus, Experimental - therapy
Disease Models, Animal
Female
Islets of Langerhans - physiology
Islets of Langerhans Transplantation - methods
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Surgery
Transplant Donor Site
Transplantation, Heterologous
Transplantation, Homologous
Transplantation, Isogeneic
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Summary:Abstract Background Reliable and reproducible transplantation is essential to the success of a number of particular investigations. Renal subcapsule is the most selected site for islet transplantation mainly owing to its easy access, readiness for retrieval, and possibility of reimplantation. Methods Syngeneic, allogeneic, and xenogeneic islets were transplanted into kidney capsules of Balb/C and C57BL/6J mice, and the blood glucose levels of the experimental animals were periodically monitored. Detailed procedures on mouse diabetic model and islet implantation are described. Results The values of blood glucose measured under varied transplant circumstances are presented, covering syngeneic, allogeneic, and xenogeneic islet transplantations. The methodology is straightforward and has been proven to be practicable and reproducible. Conclusions The parallel observations in different transplant situations provide a valuable contribution to and useful information for diabetes-related studies.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2016.06.045