Lymphoblast-derived integration-free iPSC lines from a female and male Alzheimer's disease patient expressing different copy numbers of a coding CNV in the Alzheimer risk gene CR1

Human lymphoblast cells from a female and male patient diagnosed with Alzheimer's disease (AD) with different genotypes of a functional copy number variation (CNV) in the AD risk gene CR1 were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal plasmids e...

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Bibliographic Details
Published in:Stem cell research 2016-11, Vol.17 (3), p.560-563
Main Authors: Schröter, Friederike, Sleegers, Kristel, Van Cauwenberghe, Caroline, Bohndorf, Martina, Wruck, Wasco, Van Broeckhoven, Christine, Adjaye, James
Format: Article
Language:English
Subjects:
Alzheimer Disease - genetics
Alzheimer Disease - pathology
Cell Differentiation
Cell Line
Cellular Reprogramming
DNA Copy Number Variations
DNA Fingerprinting
Embryoid Bodies - cytology
Embryoid Bodies - metabolism
Female
Gene Dosage
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Lymphocytes - cytology
Male
Microscopy, Fluorescence
Plasmids - genetics
Plasmids - metabolism
Receptors, Complement 3b - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Human lymphoblast cells from a female and male patient diagnosed with Alzheimer's disease (AD) with different genotypes of a functional copy number variation (CNV) in the AD risk gene CR1 were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. The iPSCs retained the CR1 CNV, and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearson correlation of 0.956 for AD1-CR10 and 0.908 for AD1-CR14.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2016.10.003