Microbial assisted High Impact Polystyrene (HIPS) degradation

[Display omitted] •Bacterial degradation of High Impact Polystyrene (HIPS) was observed.•Degradation with Bacillus spp. showed a weight loss of 23% (w/w) of HIPS film in 30days.•Bromine release in the form of methyl bromine from decabrominated HIPS was confirmed by NMR.•Culture supernatant of Pseudo...

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Bibliographic Details
Published in:Bioresource technology 2016-08, Vol.213, p.204-207
Main Authors: Mohan, Arya J., Sekhar, Vini C., Bhaskar, Thallada, Nampoothiri, K. Madhavan
Format: Article
Language:English
Subjects:
Bacillus (bacteria)
Bacillus - metabolism
Bacillus species
Biofilms
bromination
bromine
Bromine - chemistry
Bromine - metabolism
carbon
Carbon - metabolism
chlorides
Chromatography, High Pressure Liquid
Electronic Waste
emulsions
High Impact Polystyrene
Magnetic Resonance Spectroscopy
Methyl bromine
Microscopy, Electron, Scanning
polystyrenes
Polystyrenes - chemistry
Polystyrenes - metabolism
Pseudomonas
Pseudomonas - metabolism
Pseudomonas species
Spectroscopy, Fourier Transform Infrared
technology
tetrazolium
Tetrazolium Salts
Thermogravimetry
turbidity
viability
weight loss
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Summary:[Display omitted] •Bacterial degradation of High Impact Polystyrene (HIPS) was observed.•Degradation with Bacillus spp. showed a weight loss of 23% (w/w) of HIPS film in 30days.•Bromine release in the form of methyl bromine from decabrominated HIPS was confirmed by NMR.•Culture supernatant of Pseudomonas spp. showed 97.4% reduction in turbidity of HIPS emulsion. The efficacy of newly isolated Pseudomonas and Bacillus strains to degrade brominated High Impact Polystyrene (HIPS) was investigated. Viability of these cultures while using e-plastic as sole carbon source was validated through Triphenyl Tetrazolium Chloride (TTC). Four days incubation of HIPS emulsion with Bacillus spp. showed 94% reduction in turbidity and was 97% with Pseudomonas spp. Confirmation of degradation was concluded by HPLC, NMR, FTIR, TGA and weight loss analysis. NMR spectra of the degraded film revealed the formation of aliphatic carbon chain with bromine and its release. FTIR analysis of the samples showed a reduction in CH, CO and CN groups. Surface changes in the brominated HIPS film was visualized through SEM analysis. Degradation with Bacillus spp showed a weight loss of 23% (w/w) of HIPS film in 30days.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.03.021