Expression of genes encoding cellulolytic enzymes in some Aspergillus species

Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzym...

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Bibliographic Details
Published in:Genetics and molecular research 2016-10, Vol.15 (4)
Main Authors: Mahmoud, M A, Al-Othman, M R, Abd-El-Aziz, A R M, Metwaly, H A, Mohamed, H A
Format: Article
Language:English
Subjects:
Aspergillus - enzymology
Aspergillus - genetics
Aspergillus - isolation & purification
Base Sequence
beta-Galactosidase - metabolism
Cellulase - genetics
Cellulase - metabolism
Fermentation - genetics
Gene Expression Regulation, Fungal
Genes, Fungal
Phylogeny
Species Specificity
Transcription, Genetic
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Summary:Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzymes. In this study, we assayed the gene expression and cellulolytic enzyme production of three isolates: A. niger (KSU009), A. terreus (KC462061), and A. flavus (KSU014). Two fermentation systems, submerged fermentation and biofilm fermentation (BF), were used for this purpose. Gene expression analysis by RT-PCR showed that cbhB, exo, eglA, eglB, eglC, and β-actin genes were differentially expressed in the two fermentation systems for these three isolates during enzyme production. Furthermore, the expression of all genes was found to be higher in the BF system. The six genes were not expressed in the isolates with no cellulolytic enzyme production. The isolates were identified by morphological and molecular methods, which were based on macroscopic characteristics and sequence analysis of ITS1, ITS2, and the 5.8S regions of rDNA.
ISSN:1676-5680
1676-5680
DOI:10.4238/gmr15048913