Optimized medium culture for Acidobacteria subdivision 1 strains

Members of subdivision 1 of the phylum Acidobacteria were grown at different pH values in a new medium formulation named PSYL 5, which includes sucrose as a carbon source and other compounds (such as KH2PO4 and MgSO4.7H2O). Growth rate was nearly constant at pH 5.0 and declined at pH 3–4 and 6–7. Ho...

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Bibliographic Details
Published in:FEMS microbiology letters 2016-11, Vol.363 (21), p.fnw245
Main Authors: Campanharo, João Carlos, Kielak, Anna Maria, Castellane, Tereza Cristina Luque, Kuramae, Eiko Eurya, Lemos, Eliana Gertrudes de Macedo
Format: Article
Language:English
Subjects:
Acidobacteria
Carbon
Carbon sources
Growth rate
Microbiology
pH effects
Potassium phosphate
Potassium phosphates
Sucrose
Sugar
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Summary:Members of subdivision 1 of the phylum Acidobacteria were grown at different pH values in a new medium formulation named PSYL 5, which includes sucrose as a carbon source and other compounds (such as KH2PO4 and MgSO4.7H2O). Growth rate was nearly constant at pH 5.0 and declined at pH 3–4 and 6–7. However, it was found that effects involving good carbon/nitrogen ratios and pH on the growth of the members of Acidobacteria subdivision 1 were significant, and the strongest effect of these conditions was at pH 5.0. In addition, incubation time of 48, 72, 96 and 120 h was shorter than that described previously for members of Acidobacteria subdivision 1 on solid laboratory media. New medium formulation named PSYL 5 includes sucrose, as a carbon source, and other compounds (such as KH2PO4 and MgSO4.7H2O) for members of Acidobacteria subdivision 1.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnw245