Nerolidol-loaded nanospheres prevent behavioral impairment via ameliorating Na+, K+-ATPase and AChE activities as well as reducing oxidative stress in the brain of Trypanosoma evansi-infected mice

• Published in: Naunyn-Schmiedeberg's archives of pharmacology 2017-02, Vol.390 (2), p.139-148
• Main Authors: Baldissera, Matheus D., Souza, Carine F., Grando, Thirssa H., Moreira, Karen L. S., Schafer, Andressa S., Cossetin, Luciana F., da Silva, Ana P.T., da Veiga, Marcelo L., da Rocha, Maria Izabel U. M., Stefani, Lenita M., da Silva, Aleksandro S., Monteiro, Silvia G.
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - metabolism; Animals; Antioxidants - administration & dosage; Avoidance Learning - drug effects; Behavior, Animal - drug effects; Biomedical and Life Sciences; Biomedicine; Brain - drug effects; Brain - enzymology; Brain - pathology; Catalase - metabolism; Central Nervous System Protozoal Infections - drug therapy; Central Nervous System Protozoal Infections - enzymology; Central Nervous System Protozoal Infections - parasitology; Central Nervous System Protozoal Infections - psychology; Cholinesterase Inhibitors - administration & dosage; Cognition Disorders - drug therapy; Cognition Disorders - enzymology; Cognition Disorders - parasitology; Cognition Disorders - psychology; Disease Models, Animal; Female; GPI-Linked Proteins - antagonists & inhibitors; GPI-Linked Proteins - metabolism; Memory - drug effects; Memory Disorders - drug therapy; Memory Disorders - enzymology; Memory Disorders - parasitology; Memory Disorders - psychology; Mice; Motor Activity - drug effects; Nanospheres; Neurosciences; Nootropic Agents - administration & dosage; Original Article; Oxidative Stress - drug effects; Pharmacology/Toxicology; Reaction Time - drug effects; Reactive Oxygen Species - metabolism; Sesquiterpenes - administration & dosage; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors; Sodium-Potassium-Exchanging ATPase - metabolism; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; Trypanosoma - pathogenicity; Trypanosomiasis - drug therapy; Trypanosomiasis - enzymology; Trypanosomiasis - parasitology; Trypanosomiasis - psychology
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-016-1313-8

The aim of this study was to investigate the effect of nerolidol-loaded nanospheres (N-NS) on the treatment of memory impairment caused by Trypanosoma evansi in mice, as well as oxidative stress, and Na + , K + -ATPase and acetylcholinesterase (AChE) activities in brain tissue. Animals were submitted to behavioral tasks (inhibitory avoidance task and open-field test) 4 days postinfection (PI). Reactive oxygen species (ROS) and thiobarbituric acid-reactive substance (TBARS) levels and catalase (CAT), superoxide dismutase (SOD), Na + , K + -ATPase and AChE activities were measured on the fifth-day PI. T. evansi -infected mice showed memory deficit, increased ROS and TBARS levels and SOD and AChE activities, and decreased CAT and Na + , K + -ATPase activities compared to uninfected mice. N-NS prevented memory impairment and oxidative stress parameters (except SOD activity), while free nerolidol (N-F) restored only CAT activity. Also, N-NS treatment was able to prevent alterations in Na + , K + -ATPase and AChE activities caused by T. evansi infection. A significantly negative correlation was observed between memory and ROS production ( p