Skipjack tuna (Katsuwonus pelamis) eyeball oil exerts an anti-inflammatory effect by inhibiting NF-κB and MAPK activation in LPS-induced RAW 264.7 cells and croton oil-treated mice

• Published in: International immunopharmacology 2016-11, Vol.40, p.50-56
• Main Authors: Jeong, Da-Hyun, Kim, Koth-Bong-Woo-Ri, Kim, Min-Ji, Kang, Bo-Kyeong, Ahn, Dong-Hyun
• Format: Article
• Language: English
• Subjects: Acute toxicity; Animals; Anti-inflammation; Anti-inflammatory agents; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Anti-Inflammatory Agents - toxicity; Body weight; Cell Survival - drug effects; Croton Oil; Cyclooxygenase 2 - metabolism; Cyclooxygenase-2; Cytokines; Cytokines - metabolism; Cytotoxicity; Ear - pathology; Ear edema; Edema; Edema - chemically induced; Edema - drug therapy; Edema - pathology; Eye - chemistry; Fish Oils - pharmacology; Fish Oils - therapeutic use; Fish Oils - toxicity; Inflammation; Katsuwonus pelamis; Lipopolysaccharides; Macrophages; Male; MAP kinase; Mice; Mice, Inbred ICR; Mitogen-activated protein kinases; Mitogen-Activated Protein Kinases - antagonists & inhibitors; Mitogen-Activated Protein Kinases - metabolism; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; NF-κB protein; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Nuclear factor kappa B; Oil; Proteins; RAW 264.7 Cells; Studies; Toxicity; Toxicity testing; Toxicity Tests, Acute; Tuna; Tuna eyeball oil
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2016.07.005

The effect of tuna eyeball oil (TEO) on lipopolysaccharide (LPS)-induced inflammation in macrophage cells was investigated. TEO had no cytotoxicity in cell viability as compared to the control in LPS induced RAW 264.7 cells. TEO reduced the levels of NO and pro-inflammatory cytokines by up to 50% in a dose-dependent manner. The expression of NF-κB and MAPKs as well as iNOS and COX-2 proteins was reduced by TEO, which suggests that its anti-inflammatory activity is related to the suppression of the NF-κB and MAPK signaling pathways. The rate of formation of ear edema was reduced compared to that in the control at the highest dose tested. In an acute toxicity test, no mice were killed by TEO doses of up to 5000mg/kg body weight during the two week observation period. These results suggested that TEO may have a significant effect on inflammatory factors and be a potential anti-inflammatory therapeutic. •TEO reduced the levels of NO and pro-inflammatory cytokines by up to 50% in a dose-dependent manner.•The expression of NF-κB and MAPKs as well as iNOS and COX-2 proteins was reduced by TEO.•The rate of formation of ear edema was reduced compared to that in the control at the highest dose tested.