Trans10,cis15 18:2 Isolated from Beef Fat Does Not Have the Same Anti-Adipogenic Properties as Trans10,cis12–18:2 in 3T3-L1 Adipocytes

• Published in: Lipids 2016-11, Vol.51 (11), p.1231-1239
• Main Authors: Vahmani, Payam, Meadus, William J., Rolland, David C., Duff, Pascale, Dugan, Michael E. R.
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis; Animals; Anti-Obesity Agents - analysis; Anti-Obesity Agents - metabolism; Biohydrogenation; Biomedical and Life Sciences; Cattle; cis12–18:2; cis15–18:2; Conjugated linoleic acid; Fatty acids; Fatty Acids - analysis; Fatty Acids - metabolism; Functional Food - analysis; Gas chromatography; Hydrogenation; Life Sciences; Linoleic Acids, Conjugated - analysis; Linoleic Acids, Conjugated - metabolism; Lipidology; Lipogenesis; Medical Biochemistry; Medicinal Chemistry; Mice; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Original Article; Red Meat - analysis; Trans10; Triglycerides - analysis; Triglycerides - metabolism
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-016-4192-3

During ruminal biohydrogenation of α-linolenic acid, a non-conjugated non-methylene interrupted dienoic acid is formed containing a t 10 double bond, namely t 10, c 15–18:2. The present study was designed to examine whether t 10, c 15–18:2 would exert similar anti-adipogenic effects compared to t 10, c 12–18:2 in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with 35 or 70 µM of LNA, t 10, c 12–18:2, t 10, c 15–18:2, or bovine serum albumin (BSA) vehicle control for 120 h. Cellular triacylglycerol and protein were quantified using commercial colorimetric kits. Cells were analyzed for fatty acid composition and gene expression using gas chromatography and quantitative PCR, respectively. Trans 10, cis 12–18:2 decreased ( P