Phenotypic and functional characterization of neutrophils and monocytes from patients with myelodysplastic syndrome by flow cytometry

•Functional characterization of neutrophils by flow cytometry in MDS is feasible.•Heterogeneous of neutrophil function and markers in MDS patients.•CD16 and CD11b degranulation correlate with established MDS prognosis scores. Myelodysplastic syndrome (MDS) is a clonal stem cell disorder frequently a...

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Published in:Cellular immunology 2016-10, Vol.308, p.19-26
Main Authors: Schmidt, Cornelia S., Aranda Lopez, Pamela, Dopheide, Jörn F., Schmidt, Frank, Theobald, Matthias, Schild, Hansjörg, Lauinger-Lörsch, Evi, Nolte, Florian, Radsak, Markus P.
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
CD11b Antigen - metabolism
Cell Degranulation
Cell Separation
Effector functions
Female
Flow Cytometry
Humans
Immunophenotyping
Male
Middle Aged
Monitoring, Immunologic
Monitoring, Physiologic
Monocytes
Monocytes - immunology
Myelodysplastic syndrome
Myelodysplastic Syndromes - diagnosis
Myelodysplastic Syndromes - immunology
Neutrophils
Neutrophils - immunology
Phenotype
Prognosis
Receptors, IgG - metabolism
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Summary:•Functional characterization of neutrophils by flow cytometry in MDS is feasible.•Heterogeneous of neutrophil function and markers in MDS patients.•CD16 and CD11b degranulation correlate with established MDS prognosis scores. Myelodysplastic syndrome (MDS) is a clonal stem cell disorder frequently associated with inefficient granulopoiesis showing dysplastic polymorphonuclear neutrophils (PMNs). To assess PMN functionality in MDS in a clinical routine setting, 30 MDS patients and ten healthy volunteers were analyzed for PMN and monocyte phenotype and function (degranulation, CD62L shedding, oxidative burst and phagocytosis) upon stimulation with lipopolysaccharide by multi-color flow cytometry (MCFC). Our data show a heterogeneous pattern for CD66, CD16 and CD64 expression on PMNs of MDS patients. CD62L shedding rate and CD66 degranulation were reduced. Interestingly, we detected correlations between the WHO adapted prognostic scoring system (WPSS) and CD16 expression on PMNs as well as the international prognostic scoring system (IPSS) and CD11b degranulation by MCFC, suggesting clinical relevance of MCFC based function testing. In conclusion, MCFC of myelodysplastic immunophenotypes and PMN functionality are applicable in clinical settings, but further prospective studies are needed to assess the practical clinical value of such analyses.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2016.07.005