Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

• Published in: Virology (New York, N.Y.) N.Y.), 2016-10, Vol.497, p.111-124
• Main Authors: Chen, Zhenhai, Yuan, Fangfeng, Li, Yanhua, Shang, Pengcheng, Schroeder, Robin, Lechtenberg, Kelly, Henningson, Jamie, Hause, Benjamin, Bai, Jianfa, Rowland, Raymond R.R, Clavijo, Alfonso, Fang, Ying
• Format: Article
• Language: English
• Subjects: Adaptive Immunity; Animals; Antibodies, Viral - immunology; Base Sequence; Cell Line; Cloning, Molecular; Cytokines - metabolism; DNA, Complementary - chemistry; DNA, Complementary - genetics; EGFP reporter virus; Gene Expression; Genes, Reporter; Genetic Vectors - genetics; Genome, Viral; Immunity, Innate; Infectious clone; Infectious Disease; Mutation; Phenotype; Picornaviridae - genetics; Picornaviridae - immunology; Picornaviridae Infections - veterinary; Picornavirus infection; Recombination, Genetic; Reverse genetics; RNA, Viral; Senecavirus A; SVA pathogenesis; Swine; Swine Diseases - diagnosis; Swine Diseases - immunology; Swine Diseases - metabolism; Swine Diseases - virology; Vesicular disease; Vesicular lesion
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2016.07.003

Abstract A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.