Molecular analysis of lungs from pigs immunized with a mutant transferrin binding protein B-based vaccine and challenged with Haemophilus parasuis

• Published in: Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2016-10, Vol.48, p.69-78
• Main Authors: Martínez-Martínez, S, Rodríguez-Ferri, E.F, Frandoloso, R, Garrido-Pavón, J.J, Zaldívar-López, S, Barreiro-Méndez, C, Gutiérrez-Martín, C.B
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Animals; Bacterial Proteins - immunology; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - immunology; Cytokines - genetics; Glässer’s disease; Haemophilus parasuis; Haemophilus parasuis - genetics; Haemophilus parasuis - immunology; Immunization; Infectious Disease; Inflammation - genetics; Lung - immunology; Lung - microbiology; Mass Spectrometry; Microarray; Mutant transferrin binding protein B; Mutation; Proteomics; Quantitative real-time PCR; Real-Time Polymerase Chain Reaction; Subunit vaccine; Swine; Swine Diseases - immunology; Swine Diseases - microbiology; Swine Diseases - prevention & control; Tissue Array Analysis; Transferrin-Binding Protein B - genetics; Transferrin-Binding Protein B - immunology; Vaccination; Vaccines, Subunit - administration & dosage; Vaccines, Subunit - immunology
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2016.08.005

Highlights • Inflammatory response genes were seen in Glässer’s disease by microarray and qPCR. • Lower expression of them was seen in pigs vaccinated with an H. parasuis mutant TbpB. • Cell damage genes were seen in Glässer’s disease by microarray and qPCR. • Lower expression of them was seen in pigs vaccinated with an H. parasuis mutant TbpB. • Gene and protein expression was similar in mutant TbpB vaccinated and healthy pigs