Loading…

Effect of crowding, temperature and age on glia activation and dopaminergic neurotoxicity induced by MDMA in the mouse brain

[Display omitted] ⿢MDMA induces glia activation and dopaminergic neurotoxicity in the mouse brain.⿢The administration setting influences these effects of MDMA.⿢Exposure to crowded environments amplifies MDMA effects on glia and neurotoxicity.⿢Exposure to a high environmental temperature amplifies MD...

Full description

Saved in:
Bibliographic Details
Published in:Neurotoxicology (Park Forest South) 2016-09, Vol.56, p.127-138
Main Authors: Frau, Lucia, Simola, Nicola, Porceddu, Pier Francesca, Morelli, Micaela
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] ⿢MDMA induces glia activation and dopaminergic neurotoxicity in the mouse brain.⿢The administration setting influences these effects of MDMA.⿢Exposure to crowded environments amplifies MDMA effects on glia and neurotoxicity.⿢Exposure to a high environmental temperature amplifies MDMA effects on glia only.⿢This study is relevant to human MDMA use, often featuring crowded/hot environments. 3,4-methylenedyoxymethamphetamine (MDMA or ⿿ecstasy⿿), a recreational drug of abuse, can induce glia activation and dopaminergic neurotoxicity. Since MDMA is often consumed in crowded environments featuring high temperatures, we studied how these factors influenced glia activation and dopaminergic neurotoxicity induced by MDMA. C57BL/6J adolescent (4 weeks old) and adult (12 weeks old) mice received MDMA (4ÿ20mg/kg) in different conditions: 1) while kept 1, 5, or 10ÿcage at room temperature (21°C); 2) while kept 5ÿcage at either room (21°C) or high (27°C) temperature. After the last MDMA administration, immunohistochemistry was performed in the caudate-putamen for CD11b and GFAP, to mark microglia and astroglia, and in the substantia nigra pars compacta for tyrosine hydroxylase, to mark dopaminergic neurons. MDMA induced glia activation and dopaminergic neurotoxicity, compared with vehicle administration. Crowding (5 or 10 miceÿcage) amplified MDMA-induced glia activation (in adult and adolescent mice) and dopaminergic neurotoxicity (in adolescent mice). Conversely, exposure to a high environmental temperature (27°C) potentiated MDMA-induced glia activation in adult and adolescent mice kept 5ÿcage, but not dopaminergic neurotoxicity. Crowding and exposure to a high environmental temperature amplified MDMA-induced hyperthermia, and a positive correlation between body temperature and activation of either microglia or astroglia was found in adult and adolescent mice. These results provide further evidence that the administration setting influences the noxious effects of MDMA in the mouse brain. However, while crowding amplifies both glia activation and dopaminergic neurotoxicity, a high environmental temperature exacerbates glia activation only.
ISSN:0161-813X
1872-9711
DOI:10.1016/j.neuro.2016.07.008