Bacillus subtilis spores as adjuvants for DNA vaccines

Abstract Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered with purified antigenic proteins. In the present study we assessed whether spores possess adjuvant properties when combined with DNA vaccines. We showed that B. subtilis spores pro...

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Bibliographic Details
Published in:Vaccine 2015-05, Vol.33 (20), p.2328-2334
Main Authors: Aps, Luana R.M.M, Diniz, Mariana O, Porchia, Bruna F.M.M, Sales, Natiely S, Moreno, Ana Carolina R, Ferreira, Luís C.S
Format: Article
Language:English
Subjects:
Adjuvants
Adjuvants, Immunologic - administration & dosage
Allergy and Immunology
Animals
Antigens
Bacillus subtilis
Bacillus subtilis - immunology
Bacillus subtilis - physiology
CD8-Positive T-Lymphocytes - immunology
Cell Line, Tumor
Colleges & universities
Cytokines
Cytokines - immunology
Cytotoxicity
Dendritic cells
Dendritic Cells - immunology
Deoxyribonucleic acid
DNA
DNA vaccine
Enzyme-Linked Immunosorbent Assay
Flow cytometry
HPV-16
Human papillomavirus
Humans
Immunization
Interferon-gamma - immunology
Laboratory animals
Lymphatic system
Lymphocytes
Male
Mice, Inbred C57BL
Papillomaviridae
Papillomavirus E7 Proteins - genetics
Papillomavirus E7 Proteins - immunology
Proteins
Software
Spores
Spores, Bacterial - immunology
Vaccines
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
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Summary:Abstract Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered with purified antigenic proteins. In the present study we assessed whether spores possess adjuvant properties when combined with DNA vaccines. We showed that B. subtilis spores promoted the activation of dendritic cells in vitro and induced migration of pro-inflammatory cells after parenteral administration to mice. Likewise, co-administration of spores with a DNA vaccine encoding the human papillomavirus type 16 (HPV-16) E7 protein enhanced the activation of antigen-specific CD8+ T cell responses in vivo . Mice immunized with the DNA vaccine admixed with spores presented a protective immunity increase to previously implanted tumor cells, capable of expressing HPV-16 oncoproteins. Finally, we observed that the adjuvant effect can vary accordingly to the number of co-administered spores which may be ascribed with the ability to induce. Collectively, the present results demonstrate for the first time that B. subtilis spores can also confer adjuvant effects to DNA vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2015.03.043