Acetylcholinesterase based biosensor for monitoring of Malathion and Acephate in food samples: A voltammetric study

•This paper describes simple procedure for the fabrication of AChE biosensor.•Advantages of this biosensor were easy to construct and it operates at low cost.•This biosensor requires no preconcentration or extraction steps.•AChE biosensor was applied for detection of ML and AP in food samples. Acety...

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Bibliographic Details
Published in:Food chemistry 2014-01, Vol.142, p.188-196
Main Authors: Raghu, P., Madhusudana Reddy, T., Reddaiah, K., Kumara Swamy, B.E., Sreedhar, M.
Format: Article
Language:English
Subjects:
Acetylcholinesterase - chemistry
Acetylthiocholine chloride
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensor
Carbon paste electrode
Edible Grain - chemistry
Enzymes, Immobilized - chemistry
Food Contamination - analysis
Fruit - chemistry
Immobilisation
Malathion - analysis
Malathion and Acephate
Medical sciences
Organothiophosphorus Compounds - analysis
Pesticides - analysis
Pesticides, fertilizers and other agrochemicals toxicology
Phosphoramides
Toxicology
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Summary:•This paper describes simple procedure for the fabrication of AChE biosensor.•Advantages of this biosensor were easy to construct and it operates at low cost.•This biosensor requires no preconcentration or extraction steps.•AChE biosensor was applied for detection of ML and AP in food samples. Acetylcholinesterase (AChE) biosensor was developed through silica sol–gel (SiSG) immobilisation of AChE on the carbon paste electrode (CPE) and used as working electrode. AChE catalyses the cleavage of acetylthiocholine chloride (ASChCl or substrate) to thiocholine, which was oxidised to give a disulphide compound by dimerisation at 0.60V versus saturated calomel electrode. All the experiments were carried out in 0.1M phosphate buffer solution (PBS) at pH 7.0 and 0.1M KCl solution at room temperature. The limit of detection and limit of quantification values were found to be 0.058ppm, 0.044ppm and 0.194ppm, 0.147ppm for Malathion and Acephate, respectively. The response of the biosensor showed a good linearity range with an incubation time of 4min for Malathion and Acephate, respectively. This biosensor was used for the direct determination of pesticides without any pretreatment and it requires less time for analysis.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2013.07.047