A Simple and Facile Glucose Biosensor Based on Prussian Blue Modified Graphite String

This work describes the string sensor for the simple and sensitive detection of glucose which is based on Prussian blue (PB) modified graphite utilizing dipping. First, PB modified graphite (PB-G) strings are characterized by physical and electrochemical techniques to optimize the PB-G layer thickne...

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Published in:Journal of sensors 2016-01, Vol.2016 (2016), p.1-6
Main Authors: Lee, Gi-Ja, Park, Hun-Kuk, Chung, Joo-Ho, Lee, Seung Ho
Format: Article
Language:English
Subjects:
Analgesics
Aqueous solutions
Chitosan
Electrodes
Enzymes
Glucose
Graphite
Lead (metal)
Potassium
Screen printing
Sensors
Strings
Studies
Thickness
Uric acid
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cited_by cdi_FETCH-LOGICAL-a566t-df597108d58cadbb729dffa0200f2101755087fe3cb96f6a1a3063fdbf4e8a2f3
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creator Lee, Gi-Ja
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Chung, Joo-Ho
Lee, Seung Ho
description This work describes the string sensor for the simple and sensitive detection of glucose which is based on Prussian blue (PB) modified graphite utilizing dipping. First, PB modified graphite (PB-G) strings are characterized by physical and electrochemical techniques to optimize the PB-G layer thickness. Then, glucose oxidase (GOx) is immobilized on PB-G string electrode with biocompatible chitosan overlayer (Chi/GOx/PB-G). The Chi/GOx/PB-G string electrode exhibits a sensitivity of 641.3 μA·mM−1·cm−2 to glucose with a linear range of 0.03 to 1.0 mM ( R 2 = 0.9957 ) and a rapid response time (
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First, PB modified graphite (PB-G) strings are characterized by physical and electrochemical techniques to optimize the PB-G layer thickness. Then, glucose oxidase (GOx) is immobilized on PB-G string electrode with biocompatible chitosan overlayer (Chi/GOx/PB-G). The Chi/GOx/PB-G string electrode exhibits a sensitivity of 641.3 μA·mM−1·cm−2 to glucose with a linear range of 0.03 to 1.0 mM ( R 2 = 0.9957 ) and a rapid response time (&lt;3 s). Moreover, the Chi/GOx/PB-G string electrodes are less sensitive to common interference materials such as ascorbic acid, uric acid, galactose, and acetaminophen than to glucose. The Chi/GOx/PB-G string electrodes also show excellent reproducibility (&lt;5% RSD). Therefore, our Chi/GOx/PB-G string electrodes can be simple, robust, and reliable tools for glucose sensing which can avoid complicated and difficult multistep fabrication processes. In addition, we expect that they have many potential applications in fields ranging from health care to food analysis, in particular where single use is favorable.</description><identifier>ISSN: 1687-725X</identifier><identifier>EISSN: 1687-7268</identifier><identifier>DOI: 10.1155/2016/1859292</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Analgesics ; Aqueous solutions ; Chitosan ; Electrodes ; Enzymes ; Glucose ; Graphite ; Lead (metal) ; Potassium ; Screen printing ; Sensors ; Strings ; Studies ; Thickness ; Uric acid</subject><ispartof>Journal of sensors, 2016-01, Vol.2016 (2016), p.1-6</ispartof><rights>Copyright © 2016 Seung Ho Lee et al.</rights><rights>Copyright © 2016 Seung Ho Lee et al. 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1687-7268
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subjects Analgesics
Aqueous solutions
Chitosan
Electrodes
Enzymes
Glucose
Graphite
Lead (metal)
Potassium
Screen printing
Sensors
Strings
Studies
Thickness
Uric acid
title A Simple and Facile Glucose Biosensor Based on Prussian Blue Modified Graphite String
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