Characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, Spain

• Published in: Microbial ecology 2016-11, Vol.72 (4), p.813-820
• Main Authors: Lozano, Carmen, Gonzalez-Barrio, David, Camacho, Maria Cruz, Lima-Barbero, Jose Francisco, de la Puente, Javier, Höfle, Ursula, Torres, Carmen
• Format: Article
• Language: English
• Subjects: Aminoglycosides - pharmacology; Ampicillin - pharmacology; Ampicillin Resistance - genetics; Animal species; Animals; Animals, Wild - microbiology; Antibiotic resistance; Antimicrobial agents; Aquatic birds; Bacterial Proteins - genetics; Biomedical and Life Sciences; Carbon-Oxygen Ligases - genetics; Drug Resistance, Multiple, Bacterial - genetics; Ecology; Enterococcus casseliflavus; Enterococcus faecium; Enterococcus faecium - genetics; Enterococcus faecium - growth & development; Enterococcus faecium - isolation & purification; Enterococcus gallinarum; ENVIRONMENTAL MICROBIOLOGY; Falconiformes - microbiology; Feces - microbiology; Galliformes - microbiology; Genotypes; Geoecology/Natural Processes; Life Sciences; Microbial Ecology; Microbial Sensitivity Tests; Microbiology; Multilocus Sequence Typing; Natural environment; Nature Conservation; Public health; Spain; Sus scrofa - microbiology; Tetracycline - pharmacology; Tetracycline Resistance - genetics; Vancomycin - pharmacology; Vancomycin-Resistant Enterococci - genetics; Vancomycin-Resistant Enterococci - isolation & purification; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-015-0648-x

The objectives were to evaluate the presence of vancomycin-resistant enterococci with acquired (VRE-a) and intrinsic (VRE-i) resistance mechanisms in fecal samples from different wild animals, and analyze their phenotypes and genotypes of antimicrobial resistance. A total of 348 cloacal/rectal samples from red-legged partridges (127), white storks (81), red kites (59), and wild boars (81) (June 2014/February 2015) were inoculated in Slanetz-Bartley agar supplemented with vancomycin (4 µg/mL). We investigated the susceptibility to 12 antimicrobials and the presence of 19 antimicrobial resistance and five virulence genes. In addition, we performed multilocus sequence typing, detection of IS16 and studied Tn1546 structure. One VRE-a isolate was identified in one wild boar. This isolate was identified as Enterococcus faecium, harbored vanA gene included into Tn1546 (truncated with IS1542/IS1216), and belonged to the new ST993. This isolate contained the erm(A), erm(B), tet(M), dfrG, and dfrK genes. Neither element IS16 nor the studied virulence genes were detected. Ninety-six VRE-i isolates were identified (89 Enterococcus gallinarum and seven Enterococcus casseliflavus), with the following prevalence: red kites (71.2 %), white storks (46.9 %), red-legged partridges (7.9 %), and wild boars (4.9 %). Most E. gallinarum isolates showed resistance to tetracycline (66.3 %) and/or erythromycin (46.1 %). High-level resistance to aminoglycosides was present among our VRE-i isolates: kanamycin (22.9 %), streptomycin (11.5 %), and gentamicin (9.4 %). In general, VRE-i isolates of red kites showed higher rates of resistance for non-glycopeptide agents than those of other animal species. The dissemination of acquired resistance mechanisms in natural environments could have implications in the global spread of resistance with public health implications.