Cytotoxicity of Experimental Resin Composites on Mesenchymal Stem Cells Isolated from Two Oral Sources

Resin composite materials that are used to restore tooth cervical lesions associated with gingival recessions can hamper healing after root coverage surgeries. This study evaluates the in vitro cytotoxic effect of five resin composites (two commercial and three experimental) on oral mesenchymal stem...

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Bibliographic Details
Published in:Microscopy and microanalysis 2016-10, Vol.22 (5), p.1018-1033
Main Authors: Roman, Alexandra, Páll, Emöke, Moldovan, Mărioara, Rusu, Darian, Şoriţău, Olga, Feştilă, Dana, Lupşe, Mihaela
Format: Article
Language:English
Subjects:
Barium
Biological Applications
Brochures
Cell Line
Composite materials
Composite Resins - toxicity
Cytotoxicity
Dental cement
Dental Materials - toxicity
Fluorides
Humans
Laboratories
Materials Testing
Mesenchymal Stem Cells - drug effects
Polymerization
Resins
Solubility
Sorption
Stem cells
Teeth
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Summary:Resin composite materials that are used to restore tooth cervical lesions associated with gingival recessions can hamper healing after root coverage surgeries. This study evaluates the in vitro cytotoxic effect of five resin composites (two commercial and three experimental) on oral mesenchymal stem cells (MSCs) and the persistence of stemness properties in high passage MSCs. Sorption and solubility tests were made for all materials. MSCs were isolated from re-entry palatal and periodontal granulation tissues and were characterized and cultured on composite discs. Cytotoxicity of the materials was evaluated by the Alamar Blue viability test, by Paul Karl Horan (PKH) labeling, and by immunocytochemical staining for actin. Water and saliva sorption and solubility data revealed that two of the experimental materials behaved comparable with the marketed resin composites. The Alamar Blue viability test shows that both cell lines grew well on composite discs that seemed to induce no apparent toxic effects. No signs of disruption of cytoskeleton organization was seen. Experimental resin composites can be recommended for further investigation for obtaining approval for use. The standard minimal criteria were fulfilled for high passage MSCs. Palatal tissue regains its regenerative properties in terms of MSC presence in the re-entry area after 6 months of healing.
ISSN:1431-9276
1435-8115
DOI:10.1017/S1431927616011624