Phage display peptide libraries in molecular allergology: from epitope mapping to mimotope‐based immunotherapy

Identification of allergen epitopes is a key component in proper understanding of the pathogenesis of type I allergies, for understanding cross‐reactivity and for the development of mimotope immunotherapeutics. Phage particles have garnered recognition in the field of molecular allergology due to th...

Full description

Saved in:
Bibliographic Details
Published in:Allergy (Copenhagen) 2016-11, Vol.71 (11), p.1526-1532
Main Authors: Luzar, J., Štrukelj, B., Lunder, M.
Format: Article
Language:English
Subjects:
Allergens - chemistry
Allergens - immunology
Animals
Cell Surface Display Techniques
Cross Reactions - immunology
Epitope Mapping - methods
Humans
Hypersensitivity - diagnosis
Hypersensitivity - immunology
Hypersensitivity - therapy
Immunodominant Epitopes - chemistry
Immunodominant Epitopes - immunology
Immunoglobulin E - immunology
Immunotherapy
Libraries
mimotopes
Peptide Library
Peptides
phage display peptide libraries
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Identification of allergen epitopes is a key component in proper understanding of the pathogenesis of type I allergies, for understanding cross‐reactivity and for the development of mimotope immunotherapeutics. Phage particles have garnered recognition in the field of molecular allergology due to their value not only in competitive immunoscreening of peptide libraries but also as immunogenic carriers of allergen mimotopes. They integrate epitope discovery technology and immunization functions into a single platform. This article provides an overview of allergen mimotopes identified through the phage display technique. We discuss the contribution of phage display peptide libraries in determining dominant B‐cell epitopes of allergens, in developing mimotope immunotherapy, in understanding cross‐reactivity, and in determining IgE epitope profiles of individual patients to improve diagnostics and individualize immunotherapy. We also discuss the advantages and pitfalls of the methodology used to identify and validate the mimotopes.
ISSN:0105-4538
1398-9995
DOI:10.1111/all.12965