Light-induced Notch activity controls neurogenic and gliogenic potential of neural progenitors

Oscillations in Notch signaling are essential for reserving neural progenitors for cellular diversity in developing brains. Thus, steady and prolonged overactivation of Notch signaling is not suitable for generating neurons. To acquire greater temporal control of Notch activity and mimic endogenous...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2016-10, Vol.479 (4), p.820-826
Main Authors: Kim, Kyung-Tai, Song, Mi-Ryoung
Format: Article
Language:English
Subjects:
Animals
Astrocytes - cytology
Astrocytes - metabolism
Astrocytes - radiation effects
Cell Differentiation - radiation effects
Cell Line
Light
Mice
Mice, Inbred C57BL
Neural progenitors
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Neural Stem Cells - radiation effects
Neurogenesis - physiology
Neurogenesis - radiation effects
Neuroglia - cytology
Neuroglia - metabolism
Neuroglia - radiation effects
Neurons - cytology
Neurons - metabolism
Neurons - radiation effects
Notch
Protein Domains
Receptor, Notch1 - chemistry
Receptor, Notch1 - metabolism
Receptor, Notch1 - radiation effects
Signal Transduction
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Summary:Oscillations in Notch signaling are essential for reserving neural progenitors for cellular diversity in developing brains. Thus, steady and prolonged overactivation of Notch signaling is not suitable for generating neurons. To acquire greater temporal control of Notch activity and mimic endogenous oscillating signals, here we adopted a light-inducible transgene system to induce active form of Notch NICD in neural progenitors. Alternating Notch activity saved more progenitors that are prone to produce neurons creating larger number of mixed clones with neurons and progenitors in vitro, compared to groups with no light or continuous light stimulus. Furthermore, more upper layer neurons and astrocytes arose upon intermittent Notch activity, indicating that dynamic Notch activity maintains neural progeny and fine-tune neuron-glia diversity. •Light-switchable transgene system allows spatiotemporal control of Notch activity.•Intermittent Notch activity changes neurogenic potential of neural progenitors.•Transient but not persistent Notch activity efficiently expands neural progeny and then neurons.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2016.09.124