Customized sequential multiplex PCR for accurate and early determination of invasive pneumococcal serotypes found in India

For accurate and earlier detection of invasive pneumococcal serogroup/serotypes from India, we have rearranged the African sequence of multiplex PCR provided by the Centers for Disease Control and Prevention, USA. This modified approach can successfully be adapted for earlier serotype detection of 9...

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Bibliographic Details
Published in:Journal of microbiological methods 2016-11, Vol.130, p.133-135
Main Authors: Veeraraghavan, Balaji, Jayaraman, Ranjith, John, James, Varghese, Rosemol, Neeravi, Ayyanraj, Verghese, Valsan Philip, Thomas, Kurien
Format: Article
Language:English
Subjects:
Agglutination Tests - methods
Bacterial Proteins - genetics
Base Sequence
Customized sequential multiplex PCR
DNA, Bacterial - analysis
Early Diagnosis
Humans
India
Indian serotypes
Introduced Species
Invasive pneumococcal disease
Multiplex Polymerase Chain Reaction - methods
PCV
Pneumococcal Infections - blood
Pneumococcal Infections - cerebrospinal fluid
Pneumococcal Infections - diagnosis
Pneumococcal Infections - microbiology
Pneumococcal serotyping
Prevalence
Serogroup
Serotyping - methods
Streptococcus pneumoniae
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - immunology
Streptococcus pneumoniae - isolation & purification
Streptococcus pneumoniae - pathogenicity
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Summary:For accurate and earlier detection of invasive pneumococcal serogroup/serotypes from India, we have rearranged the African sequence of multiplex PCR provided by the Centers for Disease Control and Prevention, USA. This modified approach can successfully be adapted for earlier serotype detection of 95% of the pneumococcal strains prevalent in India. •95% of invasive pneumococcal isolates circulating in India can be serogrouped/serotyped with customized SM-PCR•76% of invasive pneumococcal isolates from India can be serogrouped/serotyped within three sequential multiplex PCR assays•Customized SM-PCR is an accurate, reproducible and cheaper method for serogroup/serotyping of pneumococcal isolates compared to conventional tests like quellung and co-agglutination techniques
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2016.09.007