An alternative method to quantify 2-MIB producing cyanobacteria in drinking water reservoirs: Method development and field applications

2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R i...

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Bibliographic Details
Published in:Environmental research 2016-11, Vol.151, p.618-627
Main Authors: Chiu, Yi-Ting, Yen, Hung-Kai, Lin, Tsair-Fuh
Format: Article
Language:English
Subjects:
2-methylisoborneol (2-MIB)
2-MIB-synthesis gene (mibC gene)
Bornanes - analysis
Bornanes - metabolism
Cyanobacteria
Cyanobacteria - genetics
Cyanobacteria - growth & development
Cyanobacteria - metabolism
Drinking Water - chemistry
Drinking Water - microbiology
Genes, Bacterial
Real-time PCR
Real-Time Polymerase Chain Reaction
Taste and odor
Water Microbiology - standards
Water Resources - supply & distribution
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Summary:2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R intended specifically for the mibC gene were developed and tested. Experimental results show that the MIBS02F/R primer set was able to capture 13 2-MIB producing cyanobacterial strains grown in the laboratory, and to effectively amplify the targeted DNA region from 17 2-MIB-producing cyanobacterial strains listed in the literature. The primers were further coupled with a TaqMan probe to detect 2-MIB producers in 29 drinking water reservoirs (DWRs). The results showed statistically significant correlations between mibC genes and 2-MIB concentrations for the data from each reservoir (R2=0.413–0.998; p
ISSN:0013-9351
1096-0953
DOI:10.1016/j.envres.2016.08.034