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Storage of Dothistroma septosporum cultures
Summary Culture collections are invaluable resources for supporting current and future research activities on plant pathogens. Reliable culture storage (preservation) methods are required for these collections. To determine what methods of storage were most reliable for the pine‐needle pathogen Doth...
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| Published in: | Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie 2016-10, Vol.46 (5), p.547-550 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3689-361fd6daa71a5be64c6182ca42ae8083f405537944b8fb112ff6bff1b7b9e5f33 |
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| cites | cdi_FETCH-LOGICAL-c3689-361fd6daa71a5be64c6182ca42ae8083f405537944b8fb112ff6bff1b7b9e5f33 |
| container_end_page | 550 |
| container_issue | 5 |
| container_start_page | 547 |
| container_title | Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie |
| container_volume | 46 |
| creator | Fraser, S. Weitz, H. J. Brown, A. V. Woodward, S. |
| description | Summary
Culture collections are invaluable resources for supporting current and future research activities on plant pathogens. Reliable culture storage (preservation) methods are required for these collections. To determine what methods of storage were most reliable for the pine‐needle pathogen Dothistroma septosporum, six widely available methods for the storage of fungi were compared: agar slopes at 4°C; slopes at −20°C, mycelial plugs under sterile reverse osmosis (RO) water at 4°C, mycelial plugs under sterile mineral oil at 4°C, uncovered mycelial plugs at 4°C and mycelial plugs under sterile 10% glycerol at −80°C. Revival of cultures was assessed after 12 and 21 months. Storage of mycelial plugs under sterile RO water at 4°C and under glycerol at −80°C both had 100% culture revival at both time points and are therefore recommended for future use. The four remaining methods had revival rates below 70% at 12 months. |
| doi_str_mv | 10.1111/efp.12189 |
| format | article |
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Culture collections are invaluable resources for supporting current and future research activities on plant pathogens. Reliable culture storage (preservation) methods are required for these collections. To determine what methods of storage were most reliable for the pine‐needle pathogen Dothistroma septosporum, six widely available methods for the storage of fungi were compared: agar slopes at 4°C; slopes at −20°C, mycelial plugs under sterile reverse osmosis (RO) water at 4°C, mycelial plugs under sterile mineral oil at 4°C, uncovered mycelial plugs at 4°C and mycelial plugs under sterile 10% glycerol at −80°C. Revival of cultures was assessed after 12 and 21 months. Storage of mycelial plugs under sterile RO water at 4°C and under glycerol at −80°C both had 100% culture revival at both time points and are therefore recommended for future use. The four remaining methods had revival rates below 70% at 12 months.</description><identifier>ISSN: 1437-4781</identifier><identifier>EISSN: 1439-0329</identifier><identifier>DOI: 10.1111/efp.12189</identifier><language>eng</language><publisher>Berlin: Blackwell Publishing Ltd</publisher><ispartof>Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie, 2016-10, Vol.46 (5), p.547-550</ispartof><rights>2015 Crown copyright. © 2015 Blackwell verlag GmbH This article is published with the permission of the Controller of HMSO and the Queen's Printer for Scotland.</rights><rights>Copyright © 2016 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3689-361fd6daa71a5be64c6182ca42ae8083f405537944b8fb112ff6bff1b7b9e5f33</citedby><cites>FETCH-LOGICAL-c3689-361fd6daa71a5be64c6182ca42ae8083f405537944b8fb112ff6bff1b7b9e5f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><contributor>Klopfenstein, N.B.</contributor><contributor>Klopfenstein, N.B.</contributor><creatorcontrib>Fraser, S.</creatorcontrib><creatorcontrib>Weitz, H. J.</creatorcontrib><creatorcontrib>Brown, A. V.</creatorcontrib><creatorcontrib>Woodward, S.</creatorcontrib><title>Storage of Dothistroma septosporum cultures</title><title>Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie</title><addtitle>For. Path</addtitle><description>Summary
Culture collections are invaluable resources for supporting current and future research activities on plant pathogens. Reliable culture storage (preservation) methods are required for these collections. To determine what methods of storage were most reliable for the pine‐needle pathogen Dothistroma septosporum, six widely available methods for the storage of fungi were compared: agar slopes at 4°C; slopes at −20°C, mycelial plugs under sterile reverse osmosis (RO) water at 4°C, mycelial plugs under sterile mineral oil at 4°C, uncovered mycelial plugs at 4°C and mycelial plugs under sterile 10% glycerol at −80°C. Revival of cultures was assessed after 12 and 21 months. Storage of mycelial plugs under sterile RO water at 4°C and under glycerol at −80°C both had 100% culture revival at both time points and are therefore recommended for future use. The four remaining methods had revival rates below 70% at 12 months.</description><issn>1437-4781</issn><issn>1439-0329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kMtKxDAUhoMoOI4ufIOCG0U6Jk2ay1LGuQiDF1QUNyHtJNqxndSkReftzUzVheDZnLP4_p_DB8AhggMU5kybeoASxMUW6CGCRQxxIrY3N4sJ42gX7Hm_gBAyykUPnN411qkXHVkTXdjmtfCNs5WKvK4b62vr2irK27Jpnfb7YMeo0uuD790HD-PR_XAaz64nl8PzWZzj0BljisyczpViSKWZpiSniCe5IonSHHJsCExTzAQhGTcZQokxNDMGZSwTOjUY98Fx11s7-95q38iq8LkuS7XUtvUSccwwRgSSgB79QRe2dcvw3ZpKCE4JZ4E66ajcWe-dNrJ2RaXcSiIo19pk0CY32gJ71rEfRalX_4NyNL75ScRdIrjTn78J5d4kZZil8vFqIqdwTG_F85Mc4i8XsXxq</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Fraser, S.</creator><creator>Weitz, H. J.</creator><creator>Brown, A. V.</creator><creator>Woodward, S.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>201610</creationdate><title>Storage of Dothistroma septosporum cultures</title><author>Fraser, S. ; Weitz, H. J. ; Brown, A. V. ; Woodward, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3689-361fd6daa71a5be64c6182ca42ae8083f405537944b8fb112ff6bff1b7b9e5f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fraser, S.</creatorcontrib><creatorcontrib>Weitz, H. J.</creatorcontrib><creatorcontrib>Brown, A. V.</creatorcontrib><creatorcontrib>Woodward, S.</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fraser, S.</au><au>Weitz, H. J.</au><au>Brown, A. V.</au><au>Woodward, S.</au><au>Klopfenstein, N.B.</au><au>Klopfenstein, N.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Storage of Dothistroma septosporum cultures</atitle><jtitle>Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie</jtitle><addtitle>For. Path</addtitle><date>2016-10</date><risdate>2016</risdate><volume>46</volume><issue>5</issue><spage>547</spage><epage>550</epage><pages>547-550</pages><issn>1437-4781</issn><eissn>1439-0329</eissn><abstract>Summary
Culture collections are invaluable resources for supporting current and future research activities on plant pathogens. Reliable culture storage (preservation) methods are required for these collections. To determine what methods of storage were most reliable for the pine‐needle pathogen Dothistroma septosporum, six widely available methods for the storage of fungi were compared: agar slopes at 4°C; slopes at −20°C, mycelial plugs under sterile reverse osmosis (RO) water at 4°C, mycelial plugs under sterile mineral oil at 4°C, uncovered mycelial plugs at 4°C and mycelial plugs under sterile 10% glycerol at −80°C. Revival of cultures was assessed after 12 and 21 months. Storage of mycelial plugs under sterile RO water at 4°C and under glycerol at −80°C both had 100% culture revival at both time points and are therefore recommended for future use. The four remaining methods had revival rates below 70% at 12 months.</abstract><cop>Berlin</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/efp.12189</doi><tpages>4</tpages></addata></record> |
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| ispartof | Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie, 2016-10, Vol.46 (5), p.547-550 |
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| source | Wiley |
| title | Storage of Dothistroma septosporum cultures |
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