RYP1 gene as a target for molecular diagnosis of histoplasmosis

This study analyzed the RYP1 gene as a target for the molecular diagnosis of histoplasmosis. This assay detected fungal DNA in 13/13 blood samples from HIV/AIDS-patients with histoplasmosis. Therefore, the detection of RYP1 gene in whole blood sample is a quick and sensitive test to diagnose histopl...

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Bibliographic Details
Published in:Journal of microbiological methods 2016-11, Vol.130, p.112-114
Main Authors: Brilhante, Raimunda Sâmia Nogueira, Guedes, Glaucia Morgana de Melo, Riello, Giovanna Barbosa, Ribeiro, Joyce Fonteles, Alencar, Lucas Pereira, Bandeira, Silviane Praciano, Castelo-Branco, Débora Souza Collares Maia, Oliveira, Jonathas Sales, Freire, Janaína Maria Maia, Mesquita, Jacó Ricarte Lima de, Camargo, Zoilo Pires de, Cordeiro, Rossana de Aguiar, Rocha, Marcos Fábio Gadelha, Sidrim, José Júlio Costa
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - complications
Base Sequence
Brazil
Coinfection - diagnosis
DNA, Fungal - blood
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Gene Targeting - methods
Histoplasma - genetics
Histoplasma - isolation & purification
Histoplasmosis
Histoplasmosis - diagnosis
Histoplasmosis - microbiology
Humans
Lentivirus
Molecular diagnosis
Molecular Diagnostic Techniques - methods
Retroviridae
RYP1
Sensitivity and Specificity
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Summary:This study analyzed the RYP1 gene as a target for the molecular diagnosis of histoplasmosis. This assay detected fungal DNA in 13/13 blood samples from HIV/AIDS-patients with histoplasmosis. Therefore, the detection of RYP1 gene in whole blood sample is a quick and sensitive test to diagnose histoplasmosis. •RYP1 gene is specific for H. capsulatum.•RYP1 gene can be used as a target for the molecular identification of H. capsulatum.•PCR-based detection of RYP1 gene can be for the diagnosis of histoplasmosis.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2016.09.006