Structural diversity and antioxidant activity of condensed tannins fractionated from mangosteen pericarp

[Display omitted] ► Tannin-rich fractions with potent antioxidant activity are obtained from mangosteen. ► Highly structural heterogeneity of mangosteen tannin is revealed by LC–MS and TOF-MS. ► Fractionation improves the detection of MALDI-TOF-MS for polymeric condensed tannin. ► Mangosteen condens...

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Bibliographic Details
Published in:Food chemistry 2011-12, Vol.129 (4), p.1710-1720
Main Authors: Zhou, Hai-Chao, Lin, Yi-Ming, Wei, Shu-Dong, Tam, Nora Fung-yee
Format: Article
Language:English
Subjects:
acetates
Antioxidant activity
antioxidants
Biological and medical sciences
Condensed tannins
Degree of polymerization
ethers
Food industries
foods
Fractionation
Fundamental and applied biological sciences. Psychology
hexane
Mangosteen pericarp
pericarp
petroleum
polymerization
proanthocyanidins
procyanidins
Structure–activity
tannins
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Summary:[Display omitted] ► Tannin-rich fractions with potent antioxidant activity are obtained from mangosteen. ► Highly structural heterogeneity of mangosteen tannin is revealed by LC–MS and TOF-MS. ► Fractionation improves the detection of MALDI-TOF-MS for polymeric condensed tannin. ► Mangosteen condensed tannin with most potent antioxidant activity in 9.27mDP. The extract from mangosteen pericarp was separated into eight fractions, four by solvent extraction (hexane, FH; petroleum ether, FP; ethyl acetate, FE; water, FW) and the other four by fractionation on Sephadex LH-20 column (fractions F1, F2, F3, and F4). Phenolic compounds were identified as the major antioxidant components in each fraction. UV–VIS spectrum, reversed-phase HPLC–ESI-MS coupled with thiolysis, normal-phase HPLC–ESI-MS and MALDI-TOF-MS analyses complementally showed that the phenolic-rich fractions were condensed tannins with structural heterogeneity in monomer units, degree of polymerization and interflavan linkages. Mangosteen condensed tannins predominately contained procyanidins with a significant amount of propelargonidin but much lower signals of prodelphinidin. Eicosapentamers of condensed tannins were detected by MALDI-TOF-MS. Both B-type and A-type linkages were present. Condensed tannins fractions from mangosteen pericarp, especially fractions F3 and FE, can be explored as beneficial food antioxidants because of their high yields and potent antioxidant activities.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2011.06.036