Screening of pigmented Bacillus aquimaris SH6 from the intestinal tracts of shrimp to develop a novel feed supplement for shrimp

Aims To develop a novel feed supplement for shrimp using pigmented spore‐forming bacterial strains isolated from their gastrointestinal tracts. Methods and Results Eight pigmented Bacillus strains were selected from the isolates based on high production of heat‐stable spores, typical UV‐Vis spectra...

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Bibliographic Details
Published in:Journal of applied microbiology 2016-11, Vol.121 (5), p.1357-1372
Main Authors: Ngo, H.T., Nguyen, T.T.N., Nguyen, Q.M., Tran, A.V., Do, H.T.V., Nguyen, A.H., Phan, T.‐N., Nguyen, A.T.V.
Format: Article
Language:English
Subjects:
Animal Feed
Animals
Bacillus
Bacillus - isolation & purification
Bacillus - metabolism
carotenoid
Carotenoids - biosynthesis
Decapoda
gastrointestinal tract
Gastrointestinal Tract - microbiology
Penaeidae - growth & development
Penaeidae - metabolism
Penaeidae - microbiology
pigmented
shrimp
supplement
Xanthophylls - metabolism
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Summary:Aims To develop a novel feed supplement for shrimp using pigmented spore‐forming bacterial strains isolated from their gastrointestinal tracts. Methods and Results Eight pigmented Bacillus strains were selected from the isolates based on high production of heat‐stable spores, typical UV‐Vis spectra of produced carotenoids (400–550 nm), and free radical scavenging activity of their extracts. Of the eight strains, the red‐orange pigmented Bacillus aquimaris SH6 was selected because it showed the highest abundance in shrimp guts (70% population). Whiteleg shrimp (n = 30 per group) fed with SH6 spores, at >3 × 106 CFU g−1 pellet for 4 weeks had redder colour (score of 21–23 vs 20–22), 2·7‐fold higher astaxanthin level (0·69 vs 0·25 μg g−1 shrimp), 34% higher weight gain (7·18 vs 5·32 g shrimp−1), and 85% higher phenoloxidase activity (OD490 = 0·265 vs 0·143) than shrimp in the control group. Conclusions The result supports the potential use of B. aquimaris SH6 as a feed supplement for promoting the colourization and weight gain, and for enhancing innate immunity of whiteleg shrimp. Significance and Impact of the Study This study demonstrates that carotenoids produced by B. aquimaris SH6 can be successfully absorbed and converted to astaxanthin in whiteleg shrimp.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13274