n-3 Fatty acids modulate the mRNA expression of the Nlrp3 inflammasome and Mtor in the liver of rats fed with high-fat or high-fat/fructose diets

• Published in: Immunopharmacology and immunotoxicology 2016-09, Vol.38 (5), p.353-363
• Main Authors: Garay-Lugo, Natalia, Domínguez-Lopez, Aarón, Miliar García, Angel, Aguilar Barrera, Eliud, Gómez López, Modesto, Gómez Alcalá, Alejandro, Martínez Godinez, Maria de los Angeles, Lara-Padilla, Eleazar
• Format: Article
• Language: English
• Subjects: Animals; Dietary Fats - pharmacology; Fatty Acids, Omega-3 - pharmacology; Fructose - pharmacology; Gene Expression Regulation - drug effects; Gene Expression Regulation - immunology; high-fat diet; high-fat/fructose diet; Inflammasomes - immunology; Liver - immunology; Male; Mechanistic Target of Rapamycin Complex 1; mTOR complex; Multiprotein Complexes - immunology; n-3 PUFAs; NLR Family, Pyrin Domain-Containing 3 Protein - immunology; NLRP3 inflammasome; obesity; Rats; Rats, Sprague-Dawley; RNA, Messenger - immunology; RT-PCR; TOR Serine-Threonine Kinases - immunology
• ISSN: 0892-3973; 1532-2513
• DOI: 10.1080/08923973.2016.1208221

Context: There is evidence that n-3 polyunsaturated fatty acids (n-3-PUFAs) can inhibit mTORC1, which should potentiate autophagy and eliminate NLRP3 inflammasome activity. Objective: Evaluate the effect of a high-fat or high-fat/fructose diet with and without n-3-PUFAs on hepatic gene expression. Materials and methods: We examined the mRNA expression by RT-PCR of Mtor, Nlrp3, and other 22 genes associated with inflammation in rats livers after a 9-week diet. The dietary regimens were low-fat (control, CD), high-fat (HF), high-fat/fructose (HF-Fr), and also each of these supplemented with n-3-PUFAs (CD-n-3-PUFAs, HF-n-3-PUFAs, and HF-Fr-n-3-PUFAs). These data were processed by GeneMania and STRING databases. Results: Compared to the control, the HF group showed a significant increase (between p