Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae

Abstract PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microo...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-07, Vol.176 (1), p.51-56
Main Authors: Bottin, Arnaud, Larche, Lydie, Villalba, François, Gaulin, Elodie, Esquerré-Tugayé, Marie-Thérèse, Rickauer, Martina
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fluorescence
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Gene expression
Generalities. Techniques
Genetic transformation
Green fluorescent protein
Host plants
Lipofectin
Markers
Microbiology
Microorganisms
Mycological methods and techniques used in mycology
Mycology
Oomycete
Pathogens
Phytopathology. Animal pests. Plant and forest protection
Phytophthora
Phytophthora parasitica
Proteins
Protoplasts
Reporter gene
Tobacco
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Summary:Abstract PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13641.x