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Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae
Abstract PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microo...
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| Published in: | FEMS microbiology letters 1999-07, Vol.176 (1), p.51-56 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c2961-5ff11cd84b7967076ea15945278d2f3a52407f5151a11896600d22d2a42638883 |
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| cites | cdi_FETCH-LOGICAL-c2961-5ff11cd84b7967076ea15945278d2f3a52407f5151a11896600d22d2a42638883 |
| container_end_page | 56 |
| container_issue | 1 |
| container_start_page | 51 |
| container_title | FEMS microbiology letters |
| container_volume | 176 |
| creator | Bottin, Arnaud Larche, Lydie Villalba, François Gaulin, Elodie Esquerré-Tugayé, Marie-Thérèse Rickauer, Martina |
| description | Abstract
PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant. |
| doi_str_mv | 10.1111/j.1574-6968.1999.tb13641.x |
| format | article |
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PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1999.tb13641.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Gene expression ; Generalities. Techniques ; Genetic transformation ; Green fluorescent protein ; Host plants ; Lipofectin ; Markers ; Microbiology ; Microorganisms ; Mycological methods and techniques used in mycology ; Mycology ; Oomycete ; Pathogens ; Phytopathology. Animal pests. Plant and forest protection ; Phytophthora ; Phytophthora parasitica ; Proteins ; Protoplasts ; Reporter gene ; Tobacco</subject><ispartof>FEMS microbiology letters, 1999-07, Vol.176 (1), p.51-56</ispartof><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1999</rights><rights>1999 INIST-CNRS</rights><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2961-5ff11cd84b7967076ea15945278d2f3a52407f5151a11896600d22d2a42638883</citedby><cites>FETCH-LOGICAL-c2961-5ff11cd84b7967076ea15945278d2f3a52407f5151a11896600d22d2a42638883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1871630$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Bottin, Arnaud</creatorcontrib><creatorcontrib>Larche, Lydie</creatorcontrib><creatorcontrib>Villalba, François</creatorcontrib><creatorcontrib>Gaulin, Elodie</creatorcontrib><creatorcontrib>Esquerré-Tugayé, Marie-Thérèse</creatorcontrib><creatorcontrib>Rickauer, Martina</creatorcontrib><title>Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae</title><title>FEMS microbiology letters</title><description>Abstract
PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant.</description><subject>Biological and medical sciences</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Gene expression</subject><subject>Generalities. Techniques</subject><subject>Genetic transformation</subject><subject>Green fluorescent protein</subject><subject>Host plants</subject><subject>Lipofectin</subject><subject>Markers</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>Oomycete</subject><subject>Pathogens</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Phytophthora</subject><subject>Phytophthora parasitica</subject><subject>Proteins</subject><subject>Protoplasts</subject><subject>Reporter gene</subject><subject>Tobacco</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqVUcuO0zAUjRBIlIF_sAAhWCTYTmzHSLNAI1qQipgFrK1bx5m6pHawndJ-Hz-Go1YgIVjgTeSb87g-pyieElyRfF7vKsJEU3LJ24pIKau0ITVvSHW8Vyx-_bpfLHAt2pJgKR4Wj2LcYYwbivmi-LEKxjjUD5MPJmrjEhqDT8Y69HK1vH2FIKI74wwyxzEDovUOBTP6kExA4Dp0sAkGtIfwNQ96H1BMU3ey7g515mAGP-5nzRm5tzr4jSnHAfLEuqwAOs2C2SxtDUp-A1p7NELa-myKbren5MdtvgXI0wDRJqsBHSBUyFntkwUH5nHxoIchmieX71XxZfnu8837cv1p9eHm7brUVHJSsr4nRHdtsxGSCyy4AcJkw6hoO9rXwGiDRc8II0BIKznHuKO0o9BQXrdtW18VL866OaFvk4lJ7W2ObMjvMX6KirS14LLBGfjsD-DOT8Hl3RStCRGNrBnNqDdnVI4lxmB6NQabgzwpgtXcrtqpuUI1V6jmdtWlXXXM5OcXC4gahj6A0zb-VmgF4fW8yfUZ9t0O5vQfBmr5cc1I5rMz30_jP9jl39b7CUUizGI</recordid><startdate>199907</startdate><enddate>199907</enddate><creator>Bottin, Arnaud</creator><creator>Larche, Lydie</creator><creator>Villalba, François</creator><creator>Gaulin, Elodie</creator><creator>Esquerré-Tugayé, Marie-Thérèse</creator><creator>Rickauer, Martina</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>199907</creationdate><title>Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae</title><author>Bottin, Arnaud ; Larche, Lydie ; Villalba, François ; Gaulin, Elodie ; Esquerré-Tugayé, Marie-Thérèse ; Rickauer, Martina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2961-5ff11cd84b7967076ea15945278d2f3a52407f5151a11896600d22d2a42638883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Gene expression</topic><topic>Generalities. Techniques</topic><topic>Genetic transformation</topic><topic>Green fluorescent protein</topic><topic>Host plants</topic><topic>Lipofectin</topic><topic>Markers</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>Oomycete</topic><topic>Pathogens</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Phytophthora</topic><topic>Phytophthora parasitica</topic><topic>Proteins</topic><topic>Protoplasts</topic><topic>Reporter gene</topic><topic>Tobacco</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bottin, Arnaud</creatorcontrib><creatorcontrib>Larche, Lydie</creatorcontrib><creatorcontrib>Villalba, François</creatorcontrib><creatorcontrib>Gaulin, Elodie</creatorcontrib><creatorcontrib>Esquerré-Tugayé, Marie-Thérèse</creatorcontrib><creatorcontrib>Rickauer, Martina</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest - Health & Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bottin, Arnaud</au><au>Larche, Lydie</au><au>Villalba, François</au><au>Gaulin, Elodie</au><au>Esquerré-Tugayé, Marie-Thérèse</au><au>Rickauer, Martina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae</atitle><jtitle>FEMS microbiology letters</jtitle><date>1999-07</date><risdate>1999</risdate><volume>176</volume><issue>1</issue><spage>51</spage><epage>56</epage><pages>51-56</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1999.tb13641.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-07, Vol.176 (1), p.51-56 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_18376940 |
| source | Oxford Journals Online |
| subjects | Biological and medical sciences Fluorescence Fundamental and applied biological sciences. Psychology Fungal plant pathogens Gene expression Generalities. Techniques Genetic transformation Green fluorescent protein Host plants Lipofectin Markers Microbiology Microorganisms Mycological methods and techniques used in mycology Mycology Oomycete Pathogens Phytopathology. Animal pests. Plant and forest protection Phytophthora Phytophthora parasitica Proteins Protoplasts Reporter gene Tobacco |
| title | Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae |
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