DNA analysis of potato + Solanum brevidens somatic hybrid lines

Three somatic hybrid lines between potato (cv. While Lady line no. Ke 79, 2n = 2x = 48) + Solanum brevidens (PI 218228, 2n = 2x = 24) were evaluated using randomly amplified polymorphic DNA (RAPD) markers. The lines originated from the same callus but showed different reactions to Erwinia carotovora...

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Bibliographic Details
Published in:Euphytica 1999, Vol.105 (2), p.103-107
Main Authors: Polgar, Z, Wielgus, S.M, Horvath, S, Helgeson, J.P
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
callus
Chromosomes
Deoxyribonucleic acid
Developmental stages
DNA
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
genetic markers
genetic polymorphism
Genetic resources, diversity
Genetics and breeding of economic plants
interspecific hybridization
Pectobacterium carotovorum subsp. carotovorum
Plant material
Potatoes
random amplified polymorphic DNA technique
Solanum palustre
Solanum tuberosum
somaclonal variation
somatic hybridization
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Summary:Three somatic hybrid lines between potato (cv. While Lady line no. Ke 79, 2n = 2x = 48) + Solanum brevidens (PI 218228, 2n = 2x = 24) were evaluated using randomly amplified polymorphic DNA (RAPD) markers. The lines originated from the same callus but showed different reactions to Erwinia carotovora ssp. carotovora, the cause of potato soft rot. By the use of 48 oligomer primers producing 99 scorable bands, DNA polymorphism were detected on 7 of 12 S. brevidens chromosomes. Loss of certain DNA segments on chromosome 5, 6, 9 and 11 were observed. Some of the variations could have taken place in early callus stage of development; others may have occurred after initiation of individual shoot regeneration. The possible involvement of missing RAPD products specific to one somatic hybrid that shows decreased resistance to bacterial soft rot is discussed.[PUBLICATION ABSTRACT]
ISSN:0014-2336
1573-5060
DOI:10.1023/A:1003451327553