Chromosome damage and aneuploidy detected by interphase multicolour FISH in benzene-exposed shale oil workers

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis 1999-09, Vol.445 (2), p.155-166
• Main Authors: Marcon, F, Zijno, A, Crebelli, R, Carere, A, Veidebaum, T, Peltonen, K, Parks, R, Schuler, M, Eastmond, D
• Format: Article
• Language: English
• Subjects: Benzene; Biomonitoring; Cytogenetics; FISH; Shale oil
• ISSN: 1383-5718; 1879-3592
• DOI: 10.1016/S1383-5718(99)00122-9

A multicolour tandem-labelling fluorescence in situ hybridization (FISH) procedure was used to detect chromosome alterations in peripheral blood cells of a group of Estonian petrochemistry workers. Twelve workers employed in benzene production and five cokery workers, together with eight unexposed rural controls, were enrolled in the study. The methodology employed, based on the in situ hybridization of adjacent centromeric and pericentromeric regions, allowed the simultaneous detection of both chromosome breakage, involving damage-prone pericentromeric regions, and hyperploidy in interphase cells. Blood smears from all subjects were hybridized with chromosome 1 specific probes, in order to detect genotoxic damage in circulating lymphocytes and granulocytes. Moreover, lymphocyte cultures were established, harvested 48 h following mitogen stimulation and hybridized with the tandem chromosomes 1 and 9 probes. No significant difference in the incidence of breakage was detected in the nucleated cells of blood smears of exposed vs. control subjects. In contrast, modest but significantly increased frequencies of breakage affecting both chromosomes 1 and 9 were observed in the cultured lymphocytes of the benzene-exposed workers compared to the unexposed controls, suggesting an expression of premutagenic lesions during the S-phase in vitro. Across the entire study group, the frequencies of breakage affecting chromosomes 1 and 9 in the stimulated lymphocytes were highly intercorrelated ( p