Gonadotropin-releasing hormone in two perciform fishes: Micropogonias furnieri and Pagrus pagrus

Gonadotropin-releasing hormone (GnRH) molecular variants were characterized by gradient reverse phase high performance liquid chromatography (RP)-HPLC) from brain extracts of two perciforms with economic importance for Argentina and Uruguay. RP-HPLC fractions were tested in radioimmunoassays (RIAs)...

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Bibliographic Details
Published in:Fish physiology and biochemistry 2001-04, Vol.24 (3), p.243-246
Main Authors: Montaner, AD, Miranda, LA, Vizziano, D, Lopez, A, Okuzawa, K, Somoza, G M
Format: Article
Language:English
Subjects:
Chromatography
Economic importance
Hormones
Liquid chromatography
Micropogonias furnieri
Pagrus pagrus
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Summary:Gonadotropin-releasing hormone (GnRH) molecular variants were characterized by gradient reverse phase high performance liquid chromatography (RP)-HPLC) from brain extracts of two perciforms with economic importance for Argentina and Uruguay. RP-HPLC fractions were tested in radioimmunoassays (RIAs) with both poly-specific and specific antisera. Both species showed the presence of the same three molecular forms, immunologically and chromatographically indistinguishable from sbGnRH, cGnRH-II and sGnRH. This study supports the hypothesis that their expression is a common pattern in perciforms.
ISSN:0920-1742
1573-5168
DOI:10.1023/A:1014062415118