Kazal-type chymotrypsin inhibitor from duck pancreas

A chymotrypsin inhibitor of the Kazal-type has been isolated from duck pancreas, by affinity chromatography on immobilized chymotrypsin, gel filtration on Bio-Gel P-10 and reverse phase (RP)-HPLC. It inhibits bovine chymotrypsin Aα with an association constant ( K a) of 2.06×10 7 M −1. The complete...

Full description

Saved in:
Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2002-03, Vol.131 (3), p.499-507
Main Authors: Wilimowska-Pelc, Anna, Olichwier, Zofia, Mazurkiewicz, Aleksander, Kowalska, Jolanta, Wilusz, Tadeusz
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Chromatography, Affinity
Chromatography, High Pressure Liquid
Chymotrypsin - antagonists & inhibitors
Duck pancreas
Ducks
Electrophoresis, Polyacrylamide Gel
Humans
Kazal-type chymotrypsin inhibitor
Molecular Sequence Data
Pancreas - chemistry
Sequence Homology, Amino Acid
Trypsin Inhibitor, Kazal Pancreatic - chemistry
Trypsin Inhibitor, Kazal Pancreatic - isolation & purification
Trypsin Inhibitor, Kazal Pancreatic - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A chymotrypsin inhibitor of the Kazal-type has been isolated from duck pancreas, by affinity chromatography on immobilized chymotrypsin, gel filtration on Bio-Gel P-10 and reverse phase (RP)-HPLC. It inhibits bovine chymotrypsin Aα with an association constant ( K a) of 2.06×10 7 M −1. The complete amino acid sequence was determined after digestion of pyridylethylated inhibitor with Staphylococcus aureus V8 protease and chemical cleavage with CNBr. Duck pancreatic chymotrypsin inhibitor (DPCI) was found to be a single polypeptide chain composed of 65 amino acid residues, corresponding to a molecular mass of 7191 Da.
ISSN:1096-4959
1879-1107
DOI:10.1016/S1096-4959(02)00008-8